120-Ding-CentromericDyn
Author reviewedProject name
120-Ding-CentromericDyn
Release date
2021-09-30
Update date
-
Pre-release URL
-
Project title
Timelapse images and BDML file for quantitative information about the centromeric dynamics during mouse oocytes meiosis
License
Method summary
See details in Ding et al. (2018) Current Biology, 28(10): 1661-1669.
PubMed URL
Kind
Image data
Organism
Mus musculus
(
NCBITaxon:10090
)
Total
67983 files / 1.2 TB
Image datasets
zipped 351 files / 506.3 GB, raw image 67632 files / 720.8 GB, total 67983 files / 1.2 TB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Image datasets
50
Quantitative datasets
0
Strain
-
Datatype
centromeric dynamics
Basedon
Experiment
XYZ-scale
XY: 0.1277332 micrometer/pixel, Z: 1.5 micrometer/slice
XY: 0.0593047 micrometer/pixel, Z: 1 micrometer/slice
XY: 0.0852922 micrometer/pixel, Z: 2.7 micrometer/slice
XY: 0.0593047 micrometer/pixel, Z: 2.7 micrometer/slice
XY: 0.0593047 micrometer/pixel, Z: 0.5 micrometer/slice
XY: 0.1339138 micrometer/pixel, Z: 0.5 micrometer/slice
XY: 0.1383776 micrometer/pixel, Z: 0.5 micrometer/slice
XY: 0.0446379 micrometer/pixel, Z: 0.5 micrometer/slice
XY: 0.0461259 micrometer/pixel, Z: 0.5 micrometer/slice
XY: 0.0691888 micrometer/pixel, Z: 0.5 micrometer/slice
XY: 0.0518916 micrometer/pixel, Z: 0.5 micrometer/slice
XY: 0.0370654 micrometer/pixel, Z: 0.8 micrometer/slice
T-scale
3 minutes for each time interval
10 minutes for each time interval
20 minutes for each time interval
15 minutes for each time interval
dblink
None
External links
None
Experiment type
Immunofluorescence
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
Zeiss LSM780
Detector model
-
Objective model
-
Filter set
-
Biological Process
centromeric sister chromatid cohesion
(
GO:0070601
)
,
meiotic cell cycle
(
GO:0051321
)
Cellular Component
kinetochore
(
GO:0000776
)
,
chromosome
(
GO:0005694
)
People
Contact
Tomoya S. Kitajima
RIKEN
Center for Biosystems Dynamics Research
Laboratory for Chromosome Segregation
2-2-3, Minatojima-minamimachi, Chuo-ku, Kobe 650-0047 Japan
Contributors
Yi Ding, Masako Kaido, Elena Llano, Alberto M. Pendas, Tomoya S. Kitajima
OMERO.gallery
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
Timelapse confocal images of a fusion protein with monomeric EGFP(mEGFP-PIAS1) exhibited a kinetochore-like localization pattern in mouse oocytes at metaphase II
Immunostaining images of a fusion protein with monomeric EGFP(mEGFP-PIAS1) exhibited a kinetochore-like localization pattern in mouse oocytes at metaphase I for 12 hours
Immunostaining images of a fusion protein with monomeric EGFP(mEGFP-PIAS1) exhibited a kinetochore-like localization pattern in mouse oocytes at metaphase I for 10 hours
Immunostaining images of a fusion protein with monomeric EGFP(mEGFP-PIAS1) exhibited a kinetochore-like localization pattern in mouse oocytes at metaphase I for 4 hours
Immunostaining images of a fusion protein with monomeric EGFP(mEGFP-PIAS1) exhibited a kinetochore-like localization pattern in mouse oocytes at metaphase I for 8 hours
Immunostaining images of a fusion protein with monomeric EGFP(mEGFP-PIAS1) exhibited a kinetochore-like localization pattern in mouse oocytes at metaphase I for 9 hours
Immunostaining images of a small ubiquitin-related modifier(SUMO)2/3 signals were enriched near kinetochores in mouse oocytes at metaphase II
Immunostaining images of a small ubiquitin-related modifier(SUMO)2/3 signals were enriched near kinetochores in mouse oocytes at metaphase II
Immunostaining images of a small ubiquitin-related modifier(SUMO)2/3 signals were enriched near kinetochores in mouse oocytes at metaphase II
Immunostaining images of a small ubiquitin-related modifier(SUMO)2/3 signals were enriched near kinetochores in mouse oocytes at metaphase I
Immunostaining images of a small ubiquitin-related modifier(SUMO)2/3 signals were enriched near kinetochores in mouse oocytes at metaphase I
Mus musculus
(
NCBITaxon:10090
)
Immunostaining images of a small ubiquitin-related modifier(SUMO)2/3 signals were enriched near kinetochores in mouse oocytes at telophase I
Mus musculus
(
NCBITaxon:10090
)
Immunostaining images of a small ubiquitin-related modifier(SUMO)2/3 signals were enriched near kinetochores in mouse oocytes at telophase I
Mus musculus
(
NCBITaxon:10090
)
Immunostaining images of a small ubiquitin-related modifier(SUMO)2/3 signals were enriched near kinetochores in mouse oocytes at telophase I
Mus musculus
(
NCBITaxon:10090
)
Immunostaining images of a small ubiquitin-related modifier(SUMO)2/3 signals were enriched near kinetochores in mouse oocytes at telophase I
Immunostaining images of a small ubiquitin-related modifier(SUMO)2/3 signals were enriched near kinetochores in mouse oocytes at metaphase I
Timelapse confocal images of mNeonGreen (mNG)-fused a small ubiquitin-related modifier(SUMO)2gg signals exhibit progressively enriched at centromeres during anaphase I and telophase I in mouse oocytes
Timelapse confocal images of mNeonGreen (mNG)-fused a small ubiquitin-related modifier(SUMO)2gg signals exhibit progressively enriched at centromeres during anaphase I and telophase I in mouse oocytes
Timelapse confocal images of mouse oocytes of constitutive kinetochore component CENP-C at metaphase I (mNG-fused constructs:green, H2B-mCherry:red)
Timelapse confocal images of mouse oocytes of wild-type PIAS1 fused to CENP-C (PIAS-CENP-C) at metaphase I (mNG-fused constructs:green, H2B-mCherry:red)
Timelapse confocal images of mouse oocytes of a dominant-negative form of PIAS1 (C350S mutation in the SP-RING domain PIAS(dn)) at metaphase I (mNG-fused constructs:green, H2B-mCherry:red)
Timelapse confocal images of mouse oocytes of a dominant-negative form of PIAS1 (PIAS1(dn)) fused to CENP-C (PIAS-CENP-C) at metaphase I (mNG-fused constructs:green, H2B-mCherry:red)
Timelapse confocal images of mouse oocytes of constitutive kinetochore component CENP-C at metaphase I
Timelapse confocal images of mouse oocytes of wild-type PIAS1 fused to CENP-C (PIAS-CENP-C) at metaphase I (mNG-fused constructs:green, H2B-mCherry:red)
Timelapse confocal images of mouse oocytes of a dominant-negative form of PIAS1 (C350S mutation in the SP-RING domain PIAS(dn)) at metaphase I (mNG-fused constructs:green, H2B-mCherry:red)
Timelapse confocal images of mouse oocytes of a dominant-negative form of PIAS1 (PIAS1(dn)) fused to CENP-C (PIAS-CENP-C) at metaphase I (mNG-fused constructs:green, H2B-mCherry:red)
Timelapse confocal images of mouse oocytes at metaphase II (mNG-fused constructs:green, H2B-mCherry:red)
Immunostaining images of mouse oocytes of constitutive kinetochore component CENP-C during telophase I as control (SUMO2/3:green, kinetochores:ACA, red, DNA:blue)
Immunostaining images of mouse oocytes of wild-type PIAS1 fused to CENP-C during telophase I (SUMO2/3:green, kinetochores:ACA, red, DNA:blue)
Immunostaining images of mouse oocytes of constitutive kinetochore component CENP-C during metaphase II as control (kinetochores:ACA, green, DNA:blue )
Immunostaining images of mouse oocytes of constitutive kinetochore component CENP-C during metaphase II as control (kinetochores:ACA, green, DNA:blue )
Immunostaining images of mouse oocytes of constitutive kinetochore component CENP-C during metaphase II as control (kinetochores:ACA, green, DNA:blue )
Immunostaining images of mouse oocytes of wild-type PIAS1 fused to CENP-C during metaphase II (kinetochores:ACA, green, DNA:blue )
Immunostaining images of mouse oocytes of wild-type PIAS1 fused to CENP-C during metaphase II (kinetochores:ACA, green, DNA:blue )
Immunostaining images of mouse oocytes of wild-type PIAS1 fused to CENP-C during metaphase II (kinetochores:ACA, green, DNA:blue )
Immunostaining images of mouse oocytes of a dominant-negative form of PIAS1 (PIAS1(dn)) fused to CENP-C (PIAS-CENP-C) at metaphase II (kinetochores:ACA, green, DNA:blue)
Immunostaining images of mouse oocytes of a dominant-negative form of PIAS1 (PIAS1(dn)) fused to CENP-C (PIAS-CENP-C) at metaphase II (kinetochores:ACA, green, DNA:blue)
Immunostaining images of mouse oocytes of a dominant-negative form of PIAS1 (PIAS1(dn)) fused to CENP-C (PIAS-CENP-C) at metaphase II (kinetochores:ACA, green, DNA:blue)
Immunostaining images of mouse oocytes of a dominant-negative form of PIAS1 (PIAS1(dn)) fused to CENP-C (PIAS-CENP-C) at metaphase II (kinetochores:ACA, green, DNA:blue)
Immunostaining images of mouse oocytes of a dominant-negative form of PIAS1 (PIAS1(dn)) fused to CENP-C (PIAS-CENP-C) at metaphase II (kinetochores:ACA, green, DNA:blue)
Immunostaining images of mouse oocytes of a dominant-negative form of PIAS1 (PIAS1(dn)) fused to CENP-C (PIAS-CENP-C) at metaphase II (kinetochores:ACA, green, DNA:blue)
Immunostaining images of mouse oocytes of a dominant-negative form of PIAS1 (PIAS1(dn)) fused to CENP-C (PIAS-CENP-C) at metaphase II (kinetochores:ACA, green, DNA:blue)
Immunostaining images of mouse oocytes of a dominant-negative form of PIAS1 (PIAS1(dn)) fused to CENP-C (PIAS-CENP-C) at metaphase II (kinetochores:ACA, green, DNA:blue)
Immunostaining images of mouse oocytes of constitutive kinetochore component CENP-C during metaphase II as control (kinetochores:ACA, green, DNA:blue )
Immunostaining images of mouse oocytes of constitutive kinetochore component CENP-C during metaphase II as control (kinetochores:ACA, green, DNA:blue )
Immunostaining images of mouse oocytes of constitutive kinetochore component CENP-C during metaphase II as control (kinetochores:ACA, green, DNA:blue )
Immunostaining images of mouse oocytes of constitutive kinetochore component CENP-C during metaphase II as control (kinetochores:ACA, green, DNA:blue )
Immunostaining images of mouse oocytes of constitutive kinetochore component CENP-C during metaphase II as control (kinetochores:ACA, green, DNA:blue )
Immunostaining images of mouse oocytes of constitutive kinetochore component CENP-C during metaphase II as control (kinetochores:ACA, green, DNA:blue )
Mus musculus
(
NCBITaxon:10090
)
Immunostaining images of mouse oocytes of constitutive kinetochore component CENP-C during metaphase II as control (kinetochores:ACA, green, DNA:blue )