SSBD:database

113-Kamioka-CellDyn thumbnail

113-Kamioka-CellDyn

Author reviewed
SSBD:OMERO.gallery SSBD:OMERO
SSBD:downloads
Project name
113-Kamioka-CellDyn
Release date
2019-11-20
Update date
-
Pre-release URL
-
Project title
-
License
CC BY
Method summary
See details in Kamioka et al. (2017) Cancer Sci, 108(2): 226-235.
PubMed URL
https://www.ncbi.nlm.nih.gov/pubmed/27960041
Kind
Image data
Organism
Mus musculus ( NCBITaxon:10090 )
Total
10 files / 2.9 GB
Image datasets
zipped 5 files / 1.1 GB, raw image 5 files / 1.8 GB, total 10 files / 2.9 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Image datasets
5
Quantitative datasets
0
Strain
PMN expressing ERK biosensor and 4T1 tumor cells expressing Red fluorescent protein
Datatype
Cell dynamics, ERK activation by FRET
Basedon
Experiment
XYZ-scale
XY: 0.552 micrometer/pixel, Z: 1 micrometer/slice
XY: 0.662 micrometer/pixel, Z: 1 micrometer/slice
XY: 0.764 micrometer/pixel, Z: 1 micrometer/slice
T-scale
59.07 second for each time interval
60.33 second for each time interval
60.31 second for each time interval
60.13 second for each time interval
59.59 second for each time interval
dblink
None
External links
None
Experiment type
FRET
Microscope type
MultiPhotonMicroscopy
Acquisition mode
-
Contrast method
Fluorescence
Microscope model
Olympus FV1200MPE-BX61WI
Detector model
-
Objective model
Olympus XLPLN 25XW-MP
Filter set
BA685RIF-3
Biological Process
activation of MAPKK activity ( GO:0007555 )
Cellular Component
cell ( GO:0005623 )
People
Contact
Yuji Kamioka
Kansai Medical University
-
-
-
Contributors
Yuji Kamioka, Kanako Takakura, Kenta Sumiyama, Michiyuki Matsuda
OMERO.gallery
https://ssbd.riken.jp/omero/gallery/show_project/260/
OMERO
https://ssbd.riken.jp/omero/webclient/?show=project-260
downloads
https://ssbd.riken.jp/data/113-Kamioka-CellDyn/

Dataset List

Thumbnail
Dataset
Organism
Kind / Links
113-Kamioka-CellDyn/fig1f_ERKactivation thumbnail
fig1f_ERKactivation
Mus musculus ( NCBITaxon:10090 )
Image data omero.gallery omero
Time-lapse images of numerous polymorphonuclear cells (PMNs) infiltarting into the luns of tumor-bearing mice with ERK activation
113-Kamioka-CellDyn/fig3b_scr4T1_PMN thumbnail
fig3b_scr4T1_PMN
Mus musculus ( NCBITaxon:10090 )
Image data omero.gallery omero
Time-lapse images of tumor-bearing BALB/c mice injected with 4T1 cells expressing either scr or sh870 RNA
113-Kamioka-CellDyn/fig3c_sh8704T1_PMN thumbnail
fig3c_sh8704T1_PMN
Mus musculus ( NCBITaxon:10090 )
Image data omero.gallery omero
Time-lapse images of osteoopontin (OPN)-depleted 4T1 cells expressing sh870 injected into BALB/c mice
113-Kamioka-CellDyn/figS10_OPN_ERK thumbnail
figS10_OPN_ERK
Mus musculus ( NCBITaxon:10090 )
Image data omero.gallery omero
Time-lapse images of osteopontin (OPN)-mediated ERK activation in polymorphonuclear cells (PMN)
113-Kamioka-CellDyn/figS8_ERK_OPN thumbnail
figS8_ERK_OPN
Mus musculus ( NCBITaxon:10090 )
Image data omero.gallery omero
Time-lapse images of ERK activation in polymorphonuclear cells (PMN)