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SSBD:OMERO
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111-Kawaguchi-NPCellsDyn
Author reviewed
SSBD:OMERO.gallery
SSBD:OMERO
SSBD:downloads
Project name
111-Kawaguchi-NPCellsDyn
Release date
2019-11-20
Update date
-
Pre-release URL
-
Project title
Time-lapse and fluorescent images of collectice dynamics of neural progenitor cells (NPCs)
License
CC BY
Method summary
See details in Kawaguchi et al. (2017) Nature, 545(7654): 327-331.
PubMed URL
https://www.ncbi.nlm.nih.gov/pubmed/28403137
Kind
Image data
Quantitative data
Organism
Mus musculus
(
NCBITaxon:10090
)
Total
70 files / 24.5 GB
Image datasets
zipped 32 files / 8.8 GB, raw image 32 files / 15.7 GB, total 64 files / 24.5 GB
Quantitative datasets
zipped 2 files / 1.5 MB, raw bdml 4 files / 12.3 MB, total 6 files / 13.8 MB
Image datasets
30
Quantitative datasets
2
Strain
-
Datatype
cell dynamics
Basedon
Experiment
XYZ-scale
XY: 0.92 micrometer/pixel, Z: a.u.
XY: 0.65 micrometer/pixel, Z: a.u.
XY: 0.94 micrometer/pixel, Z: a.u.
XY: 0.33 micrometer/pixel, Z: a.u.
T-scale
5 minutes for each time interval
dblink
None
External links
None
Experiment type
-
Microscope type
FluorescenceMicroscope
Acquisition mode
Other
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Biological Process
interkinetic nuclear migration (
GO:0022027
)
Cellular Component
nucleus (
GO:0005634
)
People
Contact
Kyogo Kawaguchi
RIKEN
Center for Biosystems Dynamics Research
Nonequilibrium physics of living matter RIKEN Hakubi Research Team
2-2-3, Minatojima-minamimachi, Chuo-ku, Kobe 650-0047 Japan
Contributors
Kyogo Kawaguchi, Ryoichiro Kageyama, Masaki Sano
OMERO.gallery
https://ssbd.riken.jp/omero/gallery/show_project/259/
OMERO
https://ssbd.riken.jp/omero/webclient/?show=project-259
downloads
https://ssbd.riken.jp/data/111-Kawaguchi-NPCellsDyn/
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
SuppMovie_SV1_left
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
Time-lapse image of neural progenitor cells (NPCs) migration at low density
SuppMovie_SV1_right
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
Time-lapse image of the fluorescent signals of neural progenitor cells (NPCs) migration from H2B-mCherry (nucleus marker)at low density
SuppMovie_SV2_left
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
Time-lapse image of neural progenitor cells (NPCs) migration at high density in a wide field of view
SuppMovie_SV2_right
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
Time-lapse image of the fluorescent signals of neural progenitor cells (NPCs) migration at high density in a wide field of view
SuppMovie_SV3
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
Time-lapse image of tracking single cells using nucleus marker H2B-mCherry in the high-density culture of neural progenitor cells (NPCs)
SuppMovie_SV4_left
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
Time-lapse image of NCAM knockdown neural progenitor cells (NPCs)
SuppMovie_SV4_right
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
Time-lapse image of the fluorescent signals of NCAM knockdown neural progenitor cells (NPCs)
SuppMovie_SV5_bottom
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
Time-lapse image of neural progenitor cells (NPCs) cultured under the boundary condition of a thin lane
SuppMovie_SV5_top
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
Time-lapse image of the fluorescent signals of neural progenitor cells (NPCs) cultured under the boundary condition of a thin lane
SuppMovie_SV6_green
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
Time-lapse image of tracking single cells using Fucci marker at G2 pahse
SuppMovie_SV6_red
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
Time-lapse image of tracking single cells using Fucci marker at G1 pahse
SuppMovie_SV7_left
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
Time-lapse image of neural progenitor cells (NPCs) around a +1/2 topological defects
SuppMovie_SV7_right
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
Time-lapse image of the fluorescent signals of neural progenitor cells (NPCs) around a +1/2 topological defects
SuppMovie_SV8_left
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
Time-lapse image of neural progenitor cells (NPCs) around a -1/2 topological defects
SuppMovie_SV8_right
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
Time-lapse image of the fluorescent signals of neural progenitor cells (NPCs) around a -1/2 topological defects
extended_Fig10a_bottom
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
Phase contrast image of C2C12 cells exsisting in the -1/2 topological defects in the orderd pattern
extended_Fig10a_top
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
Phase contrast image of C2C12 cells exsisting in the +1/2 topological defects in the orderd pattern
extended_Fig7
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
Phase contrast image of neural progenitor cells (NPCs) culture in the high-density regime
extended_Fig8
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
Large field phase contrast image of myoblast cell C2C12 culture
extended_Fig9c_bottomleft
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
Phase contrast image of fixed neural progenitor cells (NPCs) around -1/2 topological defects
extended_Fig9c_bottomright
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
Immunofluorescent image of fixed neural progenitor cells (NPCs) around -1/2 topological defects
extended_Fig9c_topleft
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
Phase contrast image of fixed neural progenitor cells (NPCs) around +1/2 topological defects
extended_Fig9c_topright
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
Immunofluorescent image of fixed neural progenitor cells (NPCs) around +1/2 topological defects
fig1a_FullRaw_Ph
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
Time-lapse image of the trajectory of neclues in two-dimensional cultured neural progenitor cells (NPCs)
fig1a_FullRaw_mCh
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
Time-lapse image of the trajectory of neural progenitor cells (NPCs) in two-dimensional culture
fig2a_track1
Mus musculus
(
NCBITaxon:10090
)
Quantitative data
BDML
BDML file for quantiative information about single-cell tracking of neural progenitor clls (NPCs)
fig2a_track2
Mus musculus
(
NCBITaxon:10090
)
Quantitative data
BDML
BDML file for quantiative information about single-cell tracking of neural progenitor clls (NPCs)
fig4a_bottom
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
Time-lapse image of the +1/2 topological defects in the dense neural progenitor cells (NPCs)
fig4a_top
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
Time-lapse image of the -1/2 topological defects in the dense neural progenitor cells (NPCs)
fig4b_merge
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
Time-lapse image of the merged +1/2 and -1/2 topological defects in the dense neural progenitor cells (NPCs)
fig4g_left
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
Time-lapse image of the +1/2 and -1/2 topological defects in the fluorescent channel of neural progenitor cells (NPCs)
fig4g_right
Mus musculus
(
NCBITaxon:10090
)
Image data
omero.gallery
omero
Time-lapse image of the +1/2 and -1/2 topological defects in the fluorescent channel of neural progenitor cells (NPCs)