111-Kawaguchi-NPCellsDyn thumbnail

111-Kawaguchi-NPCellsDyn

Author reviewed
Project name
111-Kawaguchi-NPCellsDyn
Release date
2019-11-20
Update date
-
Pre-release URL
-
Project title
Time-lapse and fluorescent images of collectice dynamics of neural progenitor cells (NPCs)
License
Method summary
See details in Kawaguchi et al. (2017) Nature, 545(7654): 327-331.
PubMed URL
Kind
Image data
Quantitative data
Organism
Mus musculus ( NCBITaxon:10090 )
Total
70 files / 24.5 GB
Image datasets
zipped 32 files / 8.8 GB, raw image 32 files / 15.7 GB, total 64 files / 24.5 GB
Quantitative datasets
zipped 2 files / 1.5 MB, raw bdml 4 files / 12.3 MB, total 6 files / 13.8 MB

Image datasets
30
Quantitative datasets
2

Strain
-
Datatype
cell dynamics
Basedon
Experiment
XYZ-scale
XY: 0.92 micrometer/pixel, Z: a.u.
XY: 0.65 micrometer/pixel, Z: a.u.
XY: 0.94 micrometer/pixel, Z: a.u.
XY: 0.33 micrometer/pixel, Z: a.u.
T-scale
5 minutes for each time interval
dblink
None
External links
None

Experiment type
-
Microscope type
FluorescenceMicroscope
Acquisition mode
Other
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Biological Process
interkinetic nuclear migration ( GO:0022027 )
Cellular Component
nucleus ( GO:0005634 )
People
Contact
Kyogo Kawaguchi
RIKEN
Center for Biosystems Dynamics Research
Nonequilibrium physics of living matter RIKEN Hakubi Research Team
2-2-3, Minatojima-minamimachi, Chuo-ku, Kobe 650-0047 Japan
Contributors
Kyogo Kawaguchi, Ryoichiro Kageyama, Masaki Sano

OMERO.gallery
OMERO
downloads

Dataset List

Thumbnail
Dataset
Organism
Kind / Links
Time-lapse image of neural progenitor cells (NPCs) migration at low density

Time-lapse image of the fluorescent signals of neural progenitor cells (NPCs) migration from H2B-mCherry (nucleus marker)at low density

Time-lapse image of neural progenitor cells (NPCs) migration at high density in a wide field of view

Time-lapse image of the fluorescent signals of neural progenitor cells (NPCs) migration at high density in a wide field of view

Time-lapse image of tracking single cells using nucleus marker H2B-mCherry in the high-density culture of neural progenitor cells (NPCs)

Time-lapse image of NCAM knockdown neural progenitor cells (NPCs)

Time-lapse image of the fluorescent signals of NCAM knockdown neural progenitor cells (NPCs)

Time-lapse image of neural progenitor cells (NPCs) cultured under the boundary condition of a thin lane

Time-lapse image of the fluorescent signals of neural progenitor cells (NPCs) cultured under the boundary condition of a thin lane

Time-lapse image of tracking single cells using Fucci marker at G2 pahse

Time-lapse image of tracking single cells using Fucci marker at G1 pahse

Time-lapse image of neural progenitor cells (NPCs) around a +1/2 topological defects

Time-lapse image of the fluorescent signals of neural progenitor cells (NPCs) around a +1/2 topological defects

Time-lapse image of neural progenitor cells (NPCs) around a -1/2 topological defects

Time-lapse image of the fluorescent signals of neural progenitor cells (NPCs) around a -1/2 topological defects

Phase contrast image of C2C12 cells exsisting in the -1/2 topological defects in the orderd pattern

Phase contrast image of C2C12 cells exsisting in the +1/2 topological defects in the orderd pattern

Phase contrast image of neural progenitor cells (NPCs) culture in the high-density regime

Large field phase contrast image of myoblast cell C2C12 culture

Phase contrast image of fixed neural progenitor cells (NPCs) around -1/2 topological defects

Immunofluorescent image of fixed neural progenitor cells (NPCs) around -1/2 topological defects

Phase contrast image of fixed neural progenitor cells (NPCs) around +1/2 topological defects

Immunofluorescent image of fixed neural progenitor cells (NPCs) around +1/2 topological defects

Time-lapse image of the trajectory of neclues in two-dimensional cultured neural progenitor cells (NPCs)

Time-lapse image of the trajectory of neural progenitor cells (NPCs) in two-dimensional culture

Mus musculus ( NCBITaxon:10090 )
Quantitative data BDML
BDML file for quantiative information about single-cell tracking of neural progenitor clls (NPCs)

Mus musculus ( NCBITaxon:10090 )
Quantitative data BDML
BDML file for quantiative information about single-cell tracking of neural progenitor clls (NPCs)

Time-lapse image of the +1/2 topological defects in the dense neural progenitor cells (NPCs)

Time-lapse image of the -1/2 topological defects in the dense neural progenitor cells (NPCs)

Time-lapse image of the merged +1/2 and -1/2 topological defects in the dense neural progenitor cells (NPCs)

Time-lapse image of the +1/2 and -1/2 topological defects in the fluorescent channel of neural progenitor cells (NPCs)

Time-lapse image of the +1/2 and -1/2 topological defects in the fluorescent channel of neural progenitor cells (NPCs)