Dataset 14-Ochiai-MolDynMS2/NMP-R_dCas9_MS2
Author reviewedProject name
Release date
2016-10-03
Update date
2018-11-15
Pre-release URL
None
Project title
None
Contact name
Hiroshi Ochiai
Organization
Hiroshima University
Department
Research Center for the Mathematics on Chromatin Live Dynamics (RcMcD)
Laboratory
None
Address
Kagamiyama 1-3-1, Higashi-Hiroshima 739-8530, Japan
License
Contributors
Hiroshi Ochiai, Takeshi Sugiwara, Takashi Yamamoto
Method summary
See details in Ochiai et al. (2015) Nucleic Acids Research, 43(19): e127
PubMed URL
File or Folder
NMP-R_dCas9_MS2
Kind
Image data
Title
Time-lapse fluorescence microscopy images of mouse embryonic stem cells (NMP-R mESCs) transfected with the MS2 sigRNA expression vector of nuclease-dead Cas9 fused to the green fluorescent protein (dCas9-GFP) and MS2 coat protein fused to the tandem near-infrared red fluorescent protein (MCP-tdiRFP) signals
Description
-
Organism
Mus musculus
(
NCBITaxon:10090
)
Strain
None
Datatype
gene expression dynamics
Basedon
Experiment
LocalID
NMP-R_dCas9_MS2
XYZ-scale
XY: 0.16 micrometer/pixel, Z: 0. micrometer/slice
T-scale
2 minute for each time interval
dblink
None
External links
BDML
None
PDPML
None
Experiment type
None
Microscope type
None
Acquisition mode
None
Contrast method
None
Microscope model
None
Detector model
None
Objective model
None
Filter set
None
Sample
-
Dye
-
Clearing
-
Note0
-
Biological Process
cellular protein localization
(
GO:0034613
)
Cellular Component
-
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Sibling records
No sibling records.