Live imaging data with 6-day (HCT116) or 3.5-day durations (HeLa experiments) for analyzing cell fate and the mitotic pattern in continuous generations following whole-genome duplication. In HCT116 experiments, the chromosome was marked using H2B-mCherry. In Hela experiments, the chromosome and the tubulin were marked with H2B-mCherry and EGFP-alpha-tubulin, respectively. The mitotic pattern (with bipolar or multipolar chromosome segregation) in HCT116 experiments was distinguished based on the total nuclear number in all daughter cells. A supplemental HCT116 live imaging data set with a 5-minute interval supports this adjudgment. The cell cycle length was also measured as the interval between consecutive mitotic events. H2B-mCherry signal was used to analyze the distribution of DNA contents in mitosis, which showed a higher frequency of asymmetric chromosome distribution in multipolar chromosome segregation events than in bipolar ones.
Guang Yang, Masaya Inoko, Kaito Ogura, Sumire Ishida-Ishihara, Yuki Tsukada, Akira Funahashi, Masanao Sato, Ryota Uehara (2026) Profiling cell proliferation after whole-genome duplication in human cells., Biology open
Published in May 11, 2026 (Electronic publication in May 11, 2026, midnight )
(Abstract) Though whole-genome duplication (WGD) contributes to cancer progression, the mechanism of post-WGD cell proliferation remains unclear. Here, using 6-day live-imaging, we analyzed the proliferation dynamics of more than 150 post-WGD HCT116 cell lineages. A quantitative comparison of mitotic patterns and cell fates between proliferative and non-proliferative lineages revealed that multipolar chromosome segregation in early mitosis is a potential key factor limiting the proliferative capacity of post-WGD progenies. Multipolar chromosome segregation correlated with suppressed post-WGD cell viability, particularly when accompanied by drastic chromosome loss or when it repeatedly occurred. Tracing proliferative lineages elucidated that they proliferated mainly by imposing the risk of multipolar chromosome segregation on one of two sub-lineages that formed after the first bipolar division. Meanwhile, a considerable proportion of proliferative lineages consisted entirely of progeny of early multipolar chromosome segregation events. Our results highlight key cellular events that determine the proliferation dynamics and diversity of post-WGD progenies, providing a fundamental reference for understanding WGD-associated biological processes.
Yang, Guang, Inoko, Masaya, Ogura, Kaito, Ishida-Ishihara, Sumire, Tsukada, Yuki, Funahashi, Akira, Sato, Masanao, Uehara, Ryota (2026/01/01), Profiling cell proliferation after whole-genome duplication in human cells, bioRxiv, 2026.03.12.711482
Published in 2026/01/01
(Abstract) None