Fluorescence images of myocytes in rat hearts cryo-fixed at different contractile state
Description
The dataset provides fluorescence images of myocytes in subepicardial myocardium of Langendorff-perfused rat hearts, which were rapidly-frozen (RF) during different contractile state including systole, diastole, and ventricular fibrillation (VF). The RF hearts were subsequently fixed with acetone and paraformaldehyde (PFA) by freeze-substitution procedure, then α-actinin in myocytes of them was immuno-stained with fluorescence labels. As control, hearts rapidly-frozen under mechanical relaxation induced by 2,3-butanedione monoxime (BDM), hearts fixed with PFA solely, hearts rapidly-frozen after PFA-fixation were also prepared.
Core Research for Evolutionary Science and Technology (CREST) fund from the Japan Science and Technology Agency (JST): JPMJCR1925, Grant-in-Aid for Early-Career Scientists from the Japan Society for the Promotion of Science: 22K18174