This dataset contains 41 light-microscopy images generated during the taxonomic characterization of Yamadazyma thunbergiae sp. nov., a yeast species associated with Bengal clock vine (Thunbergia grandiflora) and soil in Okinawa, Japan. Cells were grown on a panel of commonly used yeast media with differing nutrient compositions. The images underlie the species description reported in: Seike, T. et al. (2024) Int. J. Syst. Evol. Microbiol. 74, e006537. https://doi.org/10.1099/ijsem.0.006537.
Keywords: Yamadazyma thunbergiae; yeast; Saccharomycotina; morphology; phenotyping; Okinawa; Thunbergia grandiflora; taxonomy.
See details in Seike, et. al. (2024) Int J Syst Evol Microbiol.
Taisuke Seike, Hiroki Takekata, Natsue Sakata, Chikara Furusawa, Fumio Matsuda (2024) Yamadazyma thunbergiae sp. nov., a novel yeast species associated with Bengal clock vines and soil in Okinawa, Japan., International journal of systematic and evolutionary microbiology, Volume 74, Number 10
Published in 2024 Oct
(Abstract) Two strains, designated JCM 36746(T) and JCM 36749, were isolated from Bengal clock vine (Thunbergia grandiflora) and soil, respectively, in Okinawa, Japan. Analysis of the internal transcribed spacer (ITS) regions and D1/D2 domains of the large subunit rRNA gene sequences revealed identical sequences in both strains, indicating that they belong to the same species. Sequence analysis and physiological characterization identified these strains as representing a novel yeast species in the genus Yamadazyma. The sequence similarities of the concatenated ITS regions and D1/D2 domains indicated that JCM 36746(T) and JCM 36749 formed a well-supported distinct from closely related species belonging to the Yamadazyma clade, including Candida dendronema, C. diddensiae, C. germanica, C. kanchanaburiensis, C. naeodendra, C. vaughaniae, Y. akitaensis, Y. koratensis, Y. nakazawae, Y. philogaea, Y. phyllophila, Y. siamensis, Y. ubonensis, and three undescribed species, comprising Candida aff. naeodendra/diddensiae Y151, Candida sp. GE19S08, and Yamadazyma sp. strain NYNU 22830. The sequences of the D1/D2 domains and ITS regions differed in nucleotide substitutions by 1.51% and 2.57% or greater, respectively, from those of the previously described and undescribed related species. In addition, the physiological characteristics of the novel species were distinct from those of the closely related described species. On the basis of these findings, we propose the name Yamadazyma thunbergiae sp. nov. to classify this species within the genus Yamadazyma. The holotype used is JCM 36746(T) (ex-type strains CBS 18614 and NBRC 116657). The MycoBank accession number is MB 853823.(MeSH Terms)