Four cell lines with different plasma membrane tensions (MCF10A, MCF7, MDA-MB-231, and PC-3 cells) adhered on the track-etched membrane. (Shiomi A. et al., Nat. commun. 2024)
Four cell lines (MCF10A, MCF7, MDA-MB-231, and PC-3 cells) were adhered on the track-etched membrane (Merck, VCTP04700) precoated with 100 µg/mL fibronectin (063-05591, FUJIFILM) at 37˚C for 1 day, fixed with 2.5% glutaraldehyde in 20 mM HEPES buffer (pH7.0) for 2 h, and processed by 5% HILEM IL1000 (Hitachi High-Tech) at room temperature for 30 min. Images were acquired using a field emission scanning electron microscope (SU8220; Hitachi High-Tech), working at an accelerating voltage of 2.0 kV with a secondary electron detector.