We performed live imaging of the nuclear shape of the upper layer neurons of the visual cortex in young and aged NexCre;SUN1-GFP mice in vivo with two-photon microscopy before, during, and after visual light exposure following the prior preparations of craniotomy on the visual cortex for time-lapse imaging. NexCre;SUN1-GFP mice expressed the GFP-fused SUN1 protein, a protein on the outer nuclear membrane, in the excitatory neurons under the control of the NeuroD6/Nex gene locus.
Keywords; time-lapse imaging, two-photon microscopy, nucleus, excitatory neuron, visual cortex, visual stimulation, aging.
See detail in Frey et al., bioRxiv, 2022