91-Konagaya-AMPKactivity
Author reviewedProject name
91-Konagaya-AMPKactivity
Project DOI
-
Release date
2018-11-14
Update date
-
Pre-release URL
-
Project title
FRET images of AMPK activity in different cells
License
Method summary
See details in Konagaya et al. (2017) Cell Rep, 21(9): 2628-2638.
Kind
Image data
Organism
Homo sapiens
(
NCBITaxon:9606
)
Mus musculus
(
NCBITaxon:10090
)
Total
2003 files / 2.2 GB
Image datasets
zipped 7 files / 722.2 MB, raw image 1996 files / 1.5 GB, total 2003 files / 2.2 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Image datasets
6
Quantitative datasets
0
Paper(s)
Strain
HeLa
Datatype
Cell dynamic
Basedon
Experiment
XYZ-scale
XY: 0.454 micrometer/pixel, Z: one slice
XY: 0.73 micrometer/pixel, Z: NA
T-scale
3 minutes for each time interval
dblink
None
External links
None
Experiment type
TimeLapse
Microscope type
FluorescenceMicroscope
Acquisition mode
Other
Contrast method
Fluorescence
Microscope model
Olympus FV1000MVE or Olympus FV1200MPE-BX61WI
Detector model
two-channel GaAsP and two multialkali
Objective model
UplanSApo 30x/1.05 silicon or UplanSApo 25x/1.05 numerical aperture
Filter set
Semrock,Rochester FF01-425/30
Biological Process
AMP activated protein kinase activity
(
GO:0004679
)
Cellular Component
nucleotide activated protein kinase complex
(
GO:0031588
)
People
Contact
Kenta Terai
Kyoto University
Graduate School of Biostudies
Laboratory of Bioimaging and Cell Signaling
Contributors
Yumi Konagaya, Kenta Terai, Yusuke Hirao, Kanako Takakura, Masamichi Imajo, Yuji Kamioka, Norio Sasaoka, Akira Kakizuka, Kenta Sumiyama, Tomoichiro Asano, Michiyuki Matsuda
OMERO.gallery
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
FRET image of HeLa cells expressing AMPKAR-EV stimulated with 10mM 2-deoxyglucose
FRET image of HeLa cells expressing AMPKAR stimulated with 10mM 2-deoxyglucose
FRET image of HeLa cells expressing AMPKAR-EV stimulated with 10mM 2-deoxyglucose
FRET image of HeLa cells expressing AMPKAR stimulated with 10mM 2-deoxyglucose
FRET image of FRET/CFP ratio in hepatocytes without metformin at 0 min
FRET image of FRET/CFP ratio in hepatocytes with metformin at 60 min