Description
HT1080 human sarcoma cells were transduced with lentivirus expressing a two-color fluorescent ubiquitination-based cell cycle indicator [Fucci(CA)] (Sakaue-Sawano, A. et al., Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535). The cells were subjected to time-lapse imaging using KEYENCE BZ-X800 with a PlanFluor ×20 objective lens (NA = 0.45, WD = 8.80–7.50 mm, Ph1; KEYENCE). In KEYENCE BZ-X810, Fucci mCherry and mVenus signals were detected using a TRITC filter (Ex, 545/25 nm; Em, 605/70 nm; KEYENCE) and mVenus filter (Ex, 500/20; Em, 535/30 nm; M SQUARE), respectively. 67.3 um x 67.3 um images were acquired every 15 min for 76.5 hours.