339-Tsukita-Cobl
Author reviewedTime lapse images of cytoskeleton and cordon bleu, a microtubule-associated protein
Project ID
339-Tsukita-Cobl
Project DOI
-
Title
Time lapse images of cytoskeleton and cordon bleu, a microtubule-associated protein
Description
Liquid-liquid phase separation (LLPS) is involved in various dynamic biological phenomena. In epithelial cells, dynamic regulation of junctional actin filaments tethered to the apical junctional complex (AJC) is critical for maintaining internal homeostasis against external perturbations; however, the role of LLPS in this process remains unknown. Here, after identifying a multifunctional actin nucleator, cordon bleu (Cobl), as an AJC-en- riched microtubule-associated protein, we conducted comprehensive in vitro and in vivo analyses. We found that apical microtubules promoted LLPS of Cobl at the AJC, and Cobl actin assembly activity increased upon LLPS. Thus, microtubules spatiotemporally regulated junctional actin assembly for epithelial morphogenesis and paracellular barriers. Collectively, these findings established that LLPS of the actin nucleator Cobl mediated dynamic microtubule-actin cross-talk in junctions, which fine-tuned the epithelial barrier.
Release Date
2024-12-14
Update Date
-
License
Version
1.0.0
Method Summary
See details in Tsukita K, et. al. Sci Adv. 2023 Feb 15;9(7):eadf6358.
Kind
Image data
Total
99 files / 4.6 GB
Image datasets
zipped 42 files / 1.4 GB, raw image 57 files / 3.2 GB, total 99 files / 4.6 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Paper(s)
Organism
Mus musculus
(
NCBITaxon:10090
)
Homo sapiens
(
NCBITaxon:9606
)
Strain
-
Cell line
Eph4 mouse mammary gland epithelial cell
HEK293
(
CLO:0001230
)
Biological Process
actin filament depolymerization
(
GO:0030042
)
actin filament polymerization
(
GO:0030041
)
Cellular Component
cytosol
(
GO:0005829
)
Medical Subject Headings
-
Method involved in biological imaging
confocal microscopy
(
FBbi:00000251
)
fluorescence microscopy
(
FBbi:00000246
)
FRAP
(
FBbi:00000366
)
time lapse microscopy
(
FBbi:00000249
)
evanescent wave microscopy
(
FBbi:00000617
)
Image datasets
42
Quantitative datasets
0
OMERO.gallery
People
Contact
Atsushi Tamura
Teikyo University
Advanced Comprehensive Research Organization
-
Sachiko Tsukita
Teikyo University
Advanced Comprehensive Research Organization
-
Imaging dataset contributor
Kazuto Tsukita
(Teikyo University)
Quantitative dataset contributor
-
Datasets
Thumbnail
Dataset
Organism
Kind / Links
Time-series live imaging of Cobl clusters dynamics in Eph4 cells
Time-series FRAP live imaging of Cobl clusters on apical membrane in Eph4 cells
Mus musculus
(
NCBITaxon:10090
)
Time-series FRAP live imaging of Cobl on junction in Eph4 cells
Time-series live imaging of Cobl dynamics in HEK293 cells
Time-series images of Cobl dynamics in WT cells before and after nocodazole treatment
Time-series live imaging of EGFP-Cobl condensates in vitro
Time-series live imaging of FRAP analysis of EGFP-Cobl condensates in vitro
Time-series live imaging of half-FRAP analysis of large Cobl condensates in vitro
Time-series images of cell dynamics in WT cells before and after nocodazole treatment
Time-lapse images of the TIRF actin filaments dynamics in the absence of cobl
Time-lapse images of the TIRF actin filaments dynamics in the presence of 100 nM cobl
Time-lapse images of the TIRF actin filaments dynamics in the presence of 200 nM cobl
Time-lapse images of the TIRF actin filaments dynamics in the presence of 400 nM cobl
Time-lapse images of the TIRF actin filaments dynamics in the presence of 400 nM cobl and microtubles
Time-lapse images of the TIRF actin filament polymerization assay in the presence of cobl
Time-lapse images of the TIRF actin filament polymerization assay in the presence of cobl
Time-lapse images of the TIRF actin filament polymerization assay in the presence of cobl
Time-lapse images of the TIRF actin filament polymerization assay in the presence of cobl and MT
Time-lapse images of the TIRF actin filament polymerization assay in the presence of cobl and MT
Time-lapse images of the TIRF actin filament polymerization assay in the presence of cobl and MT
Time-lapse images of the TIRF actin filament polymerization assay in the absence of cobl
Time-lapse images of the TIRF actin filament polymerization assay in the absence of cobl
Time-lapse images of the TIRF actin filament polymerization assay in the absence of cobl
Time-lapse images of the triple-color TIRF actin filament polymerization assay
Time-lapse images of the TIRF actin filament polymerization assay in the presence of MT
Time-lapse images of the TIRF actin filament polymerization assay in the presence of MT
Time-lapse images of the TIRF actin filament polymerization assay in the presence of MT
Time-lapse images of the TIRF actin filament polymerization assay in the presence of MT and cobl
Time-lapse images of the TIRF actin filament polymerization assay in the presence of MT and cobl
Time-lapse images of the TIRF actin filament polymerization assay in the presence of MT and cobl
Time-lapse images of the TIRF actin filament polymerization assay in the absence of MT
Time-lapse images of the TIRF actin filament polymerization assay in the absence of MT
Time-lapse images of the TIRF actin filament polymerization assay in the absence of MT
Time-lapse images of the TIRF actin filament polymerization assay in the presence of coblD5
Time-lapse images of the TIRF actin filament polymerization assay in the presence of coblD5 and MT
Time-lapse images of the TIRF actin filament polymerization assay in the presence of coblFL
Time-lapse images of the TIRF actin filament polymerization assay in the presence of coblFL and MT
Time-lapse images of the TIRF actin filament polymerization assay in the absence of cobl
Time-lapse images of the TIRF actin filament polymerization assay in the absence of cobl
Time-series live imaging of Eph4 cells transiently expressing Venus-EB1 and mCherry-ZO-1
Time lapse images of CoblD5 proteins
Time lapse images of CoblFL proteins