SSBD:database

Project ID

332-Miyabara-tumor

Project DOI

-

Title

In vivo optical imaging of tumor stromal cells with hypoxia-inducible factor activity

Description

Tumors contain various stromal cells, such as immune cells, endothelial cells, and fibroblasts, which contribute to the development of a tumor-specific microenvironment characterized by hypoxia and inflammation, and are associated with malignant progression. The authors investigated the activity of intratumoral hypoxia-inducible factor (HIF), which functions as a master regulator of the cellular response to hypoxia and inflammation. They constructed the HIF activity-monitoring reporter gene hypoxia-response element-Venus-Akaluc (HVA) that expresses the green fluorescent protein Venus and modified firefly luciferase Akaluc in a HIF activity-dependent manner, and created transgenic mice harboring HVA transgene (HVA-Tg). By orthotopic transplantation of E0771, a mouse triple negative breast cancer cell line without a reporter gene, into HVA-Tg, they succeeded in noninvasively monitoring bioluminescence signals from HIF-active stromal cells as early as 8 days after transplantation. The HIF-active stromal cells initially clustered locally and then spread throughout the tumors with growth.

Release Date

2024-12-14

Update Date

-

License

CC BY-NC

Version

1.0.0

Method Summary

See details in Miyabara H, et. al. Cancer Sci. 2023 Oct;114(10):3935-3945.

Kind

Total

22 files / 31.3 MB

Image datasets

zipped 2 files / 12.2 MB, raw image 20 files / 19.0 MB, total 22 files / 31.3 MB

Quantitative datasets

zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes

Organism

Strain

Cell line

Biological Process

Cellular Component

-

Medical Subject Headings

Method involved in biological imaging