323-Kang-ZygoteElongate
Author reviewedCoordinate normalization of Arabidopsis zygote growth.
Project ID
323-Kang-ZygoteElongate
Project DOI
-
Title
Coordinate normalization of Arabidopsis zygote growth.
Description
Polarization of the zygote defines the body axis during plant development. In Arabidopsis (Arabidopsis thaliana), the zygote becomes polarized and elongates in the longitudinal direction, ultimately forming the apical–basal axis of the mature plant. Despite its importance, the mechanism for this elongation remains poorly understood. Based on live-cell imaging of the zygote, the authors developed new image analysis methods, referred to as coordinate normalization, that appropriately fix and align positions in an image, preventing fluctuation across a temporal sequence of images. Using these methods, they discovered that the zygote elongates only at its apical tip region, similar to tip-growing cells such as pollen tubes and root hairs. We also investigated the spatiotemporal dynamics of the apical tip contour of the zygote and observed that the zygote tip retains its isotropic, hemispherical apical shape during cell elongation.
Release Date
2024-11-25
Update Date
-
License
Version
1.0.0
Method Summary
See details in Kang Z, et. al. Plant Cell Physiol. 2023 Dec 6;64(11):1279-1288.
Kind
Image data
Total
4 files / 3.9 GB
Image datasets
zipped 2 files / 1001.1 MB, raw image 2 files / 2.9 GB, total 4 files / 3.9 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Paper(s)
Organism
Arabidopsis thaliana
(
NCBITaxon:3702
)
Strain
-
Cell line
-
Biological Process
zygote elongation
(
GO:0080159
)
Cellular Component
plasma membrane
(
GO:0005886
)
nucleus
(
GO:0005634
)
Medical Subject Headings
-
Method involved in biological imaging
two-photon laser scanning microscopy
(
FBbi:00000254
)
time lapse microscopy
(
FBbi:00000249
)
Image datasets
2
Quantitative datasets
0
OMERO.gallery
People
Contact
Satoru Tsugawa
Akita Prefectural University
Department of Mechanical Engineering
-
Minako Ueda
Tohoku University
Graduate School of Life Sciences
-
Imaging dataset contributor
Hikari Matsumoto
(Tohoku University)
Quantitative dataset contributor
Zichen Kang
(Akita Prefectural University)
Datasets
Thumbnail
Dataset
Organism
Kind / Links
Arabidopsis thaliana
(
NCBITaxon:3702
)
Time lapse images of the zygote after fertilization of the egg cell by the sperm cell, both of which expressed a plasma membrane marker.
Arabidopsis thaliana
(
NCBITaxon:3702
)
Time lapse images of the zygote expressing plasma membrane and nuclear markers.