SSBD:database

Project ID

305-Mochizuki-multilineRaman

Project DOI

-

Title

Raman spectral data of mouse fixed tissues and live HeLa cells using multiline illumination.

Description

Raman microscopy is an emerging tool for molecular imaging and analysis of living samples. Use of Raman microscopy in life sciences is, however, still limited because of its slow measurement speed for spectral imaging and analysis. We developed a multiline-illumination Raman microscope to achieve ultrafast Raman spectral imaging. A spectrophotometer equipped with a periodic array of confocal slits detects Raman spectra from a sample irradiated by multiple line illuminations. A comb-like Raman hyperspectral image is formed on a two-dimensional detector in the spectrophotometer, and a hyperspectral Raman image is acquired by scanning the sample with multiline illumination array. By irradiating a sample with 21 simultaneous illumination lines, we achieved high-throughput Raman hyperspectral imaging of mouse brain tissue, acquiring 1108800 spectra in 11.4 min. We also measured mouse kidney and liver tissue as well as conducted label-free live-cell molecular imaging.

Release Date

2024-11-25

Update Date

-

License

CC BY

Version

1.0.0

Method Summary

See details in Mochizuki K, et. al. Biomed Opt Express. 2023 Feb 7;14(3):1015-1026.

Kind

Total

24 files / 2.9 GB

Image datasets

zipped 8 files / 1.5 GB, raw image 16 files / 1.5 GB, total 24 files / 2.9 GB

Quantitative datasets

zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes

Organism

Strain

-

Cell line

Biological Process

-

Cellular Component

-

Medical Subject Headings

-

Method involved in biological imaging