163-Wang-MitochondrialDyn
Author reviewedTime-lapse STED imaging of mitochondrial dynamics in MitoPB Yellow-labeled cells
Project ID
163-Wang-MitochondrialDyn
Project DOI
-
Title
Time-lapse STED imaging of mitochondrial dynamics in MitoPB Yellow-labeled cells
Description
-
Release Date
2022-11-23
Update Date
-
License
Version
1.0.0
Method Summary
See details in Wang C, et. al. (2019) Proc Natl Acad Sci U S A., 116(32):15817-15822.
Kind
Image data
Total
20 files / 709.4 MB
Image datasets
zipped 10 files / 320.7 MB, raw image 10 files / 388.7 MB, total 20 files / 709.4 MB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Paper(s)
Organism
Homo sapiens
(
NCBITaxon:9606
)
Strain
-
Cell line
HeLa cell
(
CLO:0003684
)
Biological Process
mitochondrial fusion
(
GO:0008053
)
Cellular Component
mitochondrion
(
GO:0005739
)
mitochondrial crista
(
GO:0030061
)
mitochondrial inner membrane
(
GO:0005743
)
mitochondrial outer membrane
(
GO:0005741
)
mitochondrial membrane
(
GO:0031966
)
Medical Subject Headings
Cell Line
(
MeSH:D002460
)
Fluorescent Dyes
(
MeSH:D005456
)
Fluorescent Dyes/chemistry
(
MeSH:Q000737
)
Humans
(
MeSH:D006801
)
Imaging
Three-Dimensional
(
MeSH:D021621
)
Light
(
MeSH:D008027
)
Mitochondria
(
MeSH:D008928
)
Mitochondria/chemistry
(
MeSH:Q000737
)
Mitochondrial Membranes
(
MeSH:D051336
)
Mitochondrial Membranes/metabolism
(
MeSH:Q000378
)
Time-Lapse Imaging
(
MeSH:D059008
)
Method involved in biological imaging
Stimulation emission depletion
(
FBbi:00000334
)
time lapse microscopy
(
FBbi:00000249
)
Image datasets
10
Quantitative datasets
0
OMERO.gallery
People
Contact
Taki Masayasu
Nagoya University
Institute of Transformative Bio-Molecules
Functional Organic Materials Laboratory
Yasushi Okada
RIKEN
Center for Biosystems Dynamics Research
Laboratory for Cell Polarity Regulation
Shigehiro Yamaguchi
Nagoya University
Institute of Transformative Bio-Molecules
Functional Organic Materials Laboratory
Imaging dataset contributor
-
Quantitative dataset contributor
-
Datasets
Thumbnail
Dataset
Organism
Kind / Links
STED imaging of mitochondria of living HeLa cells
STED images of mitochondria
after deconvolution
STED images of mitochondria
before deconvolution.
STED images of mitochondria inner and outer membrane labeled with two-color.
Deconvoluted STED images of the mitochondrial inner membrane cultured in DMEM as control
Deconvoluted STED images of the mitochondrial inner membrane cultured in HBSS with nutrition starvation for 12h
Deconvoluted STED images of the mitochondrial inner membrane cultured in HBSS with nutrition starvation for 3h
STED image of cristae in HeLa cells pretreated with 10 μM mitochondrial DNA replication inhibitor (ddC) for 5 d
Time-lapse STED imaging of mitochondrial dynamics in
MitoPB Yellow-labeled cells
Time-lapse STED imaging of mitochondrial dynamics in
MitoPB Yellow-labeled cells