7
( Image datasets: 0,
Quantitative data datasets: 7 )
Organism(s)
D. rerio
Datatype
nuclear positions
Molecular Function (MF)
Biological Process (BP)
embryo development
Cellular Component (CC)
nucleus
Biological Imaging Method
-
Image Acquisition
ConfocalMicroscope
,
TimeLapse
,
Other
,
Other
XYZ Scale
XY: 1.0 micrometer/pixel, Z: 1.0 micrometer/frame
T scale
90 second for each time interval
Related paper(s)
Philipp J Keller, Annette D Schmidt, Joachim Wittbrodt, Ernst H K Stelzer (2008) Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy., Science (New York, N.Y.), Volume 322, Number 5904, pp. 1065-9
Published in 2008 Nov 14
(Electronic publication in Oct. 9, 2008, midnight )
(Abstract) A long-standing goal of biology is to map the behavior of all cells during vertebrate embryogenesis. We developed digital scanned laser light sheet fluorescence microscopy and recorded nuclei localization and movement in entire wild-type and mutant zebrafish embryos over the first 24 hours of development. Multiview in vivo imaging at 1.5 billion voxels per minute provides "digital embryos," that is, comprehensive databases of cell positions, divisions, and migratory tracks. Our analysis of global cell division patterns reveals a maternally defined initial morphodynamic symmetry break, which identifies the embryonic body axis. We further derive a model of germ layer formation and show that the mesendoderm forms from one-third of the embryo's cells in a single event. Our digital embryos, with 55 million nucleus entries, are provided as a resource.