Summary of 5-Keller-FishEmbryo

Title
-
Description
-
Relase date
2013-10-03
Updated date
2018-11-15
License
CC BY-NC-SA
Kind
Quantitative data based on Experiment
Number of Datasets
7 ( Image datasets: 0, Quantitative data datasets: 7 )

Organism(s)
D. rerio

Datatype
nuclear positions
Molecular Function (MF)
Biological Process (BP)
embryo development
Cellular Component (CC)
nucleus
Biological Imaging Method
-
Image Acquisition
ConfocalMicroscope , TimeLapse , Other , Other
XYZ Scale
XY: 1.0 micrometer/pixel, Z: 1.0 micrometer/frame
T scale
90 second for each time interval

Related paper(s)

Philipp J Keller, Annette D Schmidt, Joachim Wittbrodt, Ernst H K Stelzer (2008) Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy., Science (New York, N.Y.), Volume 322, Number 5904, pp. 1065-9

Published in 2008 Nov 14 (Electronic publication in Oct. 9, 2008, midnight )

(Abstract) A long-standing goal of biology is to map the behavior of all cells during vertebrate embryogenesis. We developed digital scanned laser light sheet fluorescence microscopy and recorded nuclei localization and movement in entire wild-type and mutant zebrafish embryos over the first 24 hours of development. Multiview in vivo imaging at 1.5 billion voxels per minute provides "digital embryos," that is, comprehensive databases of cell positions, divisions, and migratory tracks. Our analysis of global cell division patterns reveals a maternally defined initial morphodynamic symmetry break, which identifies the embryonic body axis. We further derive a model of germ layer formation and show that the mesendoderm forms from one-third of the embryo's cells in a single event. Our digital embryos, with 55 million nucleus entries, are provided as a resource.
(MeSH Terms)

Contact
Philipp J. Keller , European Molecular Biology Laboratory , Cell Biology and Biophysics Unit
Contributors
Philipp J. Keller, Annette D. Schmidt, Joachim Wittbrodt, Ernst H.K. Stelzer


Dataset List of 5-Keller-FishEmbryo

#
Dataset ID
Dataset Kind
4D View
SSBD:OMERO
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Dataset Kind Quantitative data
4D view
SSBD:OMERO
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# 478
Dataset Kind Quantitative data
4D view
SSBD:OMERO
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# 479
Dataset Kind Quantitative data
4D view
SSBD:OMERO
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# 480
Dataset Kind Quantitative data
4D view
SSBD:OMERO
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# 481
Dataset Kind Quantitative data
4D view
SSBD:OMERO
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# 482
Dataset Kind Quantitative data
4D view
SSBD:OMERO
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# 483
Dataset Kind Quantitative data
4D view
SSBD:OMERO
Download BDML
Download Image data