Summary of 47-Noguchi-CellDyn

SSBD:database
SSBD:database URL
Title
-
Description
-
Relase date
2018-11-14
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
38 ( Image datasets: 38, Quantitative data datasets: 0 )
Size of Datasets
90.2 GB ( Image datasets: 90.2 GB, Quantitative data datasets: 0 bytes )

Organism(s)
M. musculus
Gene symbol(s)
Ascl1, Ret
Protein name(s)
CXCR4, H2B, Laminin, N-cadherin, PGP9.5, RET
Protein tag(s)
EGFP, mCherry
Reporter(s)
DAPI

Datatype
cell dynamics
Molecular Function (MF)
Biological Process (BP)
-
Cellular Component (CC)
-
Biological Imaging Method
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XYZ Scale
XY: 0.828 micrometer/pixel, Z: 7.0 micrometer/pixel, XY: 1.656 micrometer/pixel, Z: 11.9 micrometer/pixel, XY: 0.414 micrometer/pixel, Z: 2.5 micrometer/pixel, XY: 0.489 micrometer/pixel, Z: 3.0 micrometer/pixel, XY: 0.033 micrometer/pixel, Z: 0.375 micrometer/pixel, XY: 0.828 micrometer/pixel, Z: 6.0 micrometer/pixel, XY: 0.636 micrometer/pixel, Z: 7.0 micrometer/pixel, XY: 1.136 micrometer/pixel, Z: 6.5 micrometer/pixel, XY: 1.656 micrometer/pixel, Z: 17.179 micrometer/pixel, XY: 0.196 micrometer/pixel, Z: NA, XY: 0.132 micrometer/pixel, Z: NA, XY: 0.830 micrometer/pixel, Z: NA, XY: 0.664 micrometer/pixel, Z: 2.5 micrometer/pixel, XY: 0.636 micrometer/pixel, Z: 6.0 micrometer/pixel, XY: 0.415 micrometer/pixel, Z: NA, XY: 1.325 micrometer/pixel, Z: 6.0 micrometer/pixel, XY: 0.461 micrometer/pixel, Z: NA, NA, XY: 0.496 micrometer/pixel, Z: 2.5 micrometer/pixel, XY: 1.325 micrometer/pixel, Z: 6.5 micrometer/pixel
T scale
30 minute , NA

Image Acquisition
Experiment type
Immunocytochemistry, Immunofluorescence
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
Olympus FV1200MPE-IX83, Carl Zeiss LSM710, Olympus FV1200MPE, NA
Detector model
NA, PMT
Objective model
Olympus XLPLN25XSVMP 25x/0.9, Carl Zeiss LD LCI Plan-Apochromat 63x/1.4 Oil DIC M27, Olympus XLPLN25XSVMP 25x/0.8, NA, Carl Zeiss Plan-Apochromat 20x/0.8 M27, Plan-Apochromat 63x/1.40 Oil DIC M27, Carl Zeiss LD LCI Plan-Apochromat 25x/0.8 Imm Korr DIC M27, Olympus XLPLN25XWMP 25x/1.05, Plan-Apochromat 20x/0.8
Filter set
-

Related paper(s)

Masafumi Noguchi, Kenta Sumiyama, Mitsuru Morimoto (2015) Directed Migration of Pulmonary Neuroendocrine Cells toward Airway Branches Organizes the Stereotypic Location of Neuroepithelial Bodies., Cell reports, Volume 13, Number 12, pp. 2679-86

Published in 2015 Dec 29 (Electronic publication in Dec. 17, 2015, midnight )

(Abstract) The airway epithelium consists of diverse cell types, including neuroendocrine (NE) cells. These cells are thought to function as chemoreceptors and as a component of the stem cell niche as well as the cells of origin in small-cell lung cancer. NE cells often localize at bifurcation points of airway tubes, forming small clusters called neuroepithelial bodies (NEBs). To investigate NEB development, we established methods for 3D mapping and ex vivo 4D imaging of developing lungs. We found that NEBs localize at stereotypic positions in the bifurcation area irrespective of variations in size. Notch-Hes1 signaling contributes to the differentiation of solitary NE cells, regulating their number but not localization. Live imaging revealed that individual NE cells migrate distally to and cluster at bifurcation points, driving NEB formation. We propose that NEB development is a multistep process involving differentiation of individual NE cells and their directional migration to organize NEBs.
(MeSH Terms)

Contact
Masafumi Noguchi, Mitsuru Morimoto , RIKEN , Center for Developmental Biology , Laboratory for Lung Development
Contributors
Masafumi Noguchi, Kenta Sumiyama, Mitsuru Morimoto


Dataset List of 47-Noguchi-CellDyn

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
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# 1241
Datast ID Fig1A
Dataset Kind Image data
Dataset Size 12.8 MB
4D view
SSBD:OMERO
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# 1242
Datast ID Fig1B
Dataset Kind Image data
Dataset Size 8.5 MB
4D view
SSBD:OMERO
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# 1243
Datast ID Fig1D
Dataset Kind Image data
Dataset Size 757.5 MB
4D view
SSBD:OMERO
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# 1244
Datast ID Fig1E
Dataset Kind Image data
Dataset Size 757.5 MB
4D view
SSBD:OMERO
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# 1245
Datast ID Fig1F
Dataset Kind Image data
Dataset Size 741.5 MB
4D view
SSBD:OMERO
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# 1246
Datast ID Fig2A
Dataset Kind Image data
Dataset Size 63.0 MB
4D view
SSBD:OMERO
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# 1247
Datast ID Fig2B
Dataset Kind Image data
Dataset Size 22.7 MB
4D view
SSBD:OMERO
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# 1248
Datast ID Fig2E
Dataset Kind Image data
Dataset Size 1.9 GB
4D view
SSBD:OMERO
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# 1249
Datast ID Fig3A
Dataset Kind Image data
Dataset Size 686.0 MB
4D view
SSBD:OMERO
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# 1250
Datast ID Fig3C
Dataset Kind Image data
Dataset Size 6.3 GB
4D view
SSBD:OMERO
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# 1251
Datast ID Fig3E
Dataset Kind Image data
Dataset Size 5.4 GB
4D view
SSBD:OMERO
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# 1252
Datast ID Fig3G
Dataset Kind Image data
Dataset Size 2.4 GB
4D view
SSBD:OMERO
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# 1253
Datast ID Fig3H
Dataset Kind Image data
Dataset Size 88.1 MB
4D view
SSBD:OMERO
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# 1254
Datast ID Fig4B
Dataset Kind Image data
Dataset Size 15.5 GB
4D view
SSBD:OMERO
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# 1255
Datast ID Fig4C
Dataset Kind Image data
Dataset Size 1.7 GB
4D view
SSBD:OMERO
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# 1256
Datast ID Fig4D
Dataset Kind Image data
Dataset Size 1.5 GB
4D view
SSBD:OMERO
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# 1257
Datast ID Fig4F
Dataset Kind Image data
Dataset Size 45.2 MB
4D view
SSBD:OMERO
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# 1258
Datast ID Fig4G
Dataset Kind Image data
Dataset Size 6.2 MB
4D view
SSBD:OMERO
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# 1259
Datast ID FigS1B-1
Dataset Kind Image data
Dataset Size 8.5 MB
4D view
SSBD:OMERO
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# 1260
Datast ID FigS1B-2
Dataset Kind Image data
Dataset Size 262.1 MB
4D view
SSBD:OMERO
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# 1261
Datast ID FigS1B-3
Dataset Kind Image data
Dataset Size 264.1 MB
4D view
SSBD:OMERO
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# 1262
Datast ID FigS1D
Dataset Kind Image data
Dataset Size 50.1 MB
4D view
SSBD:OMERO
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# 1263
Datast ID FigS1G-E14.5-2
Dataset Kind Image data
Dataset Size 916.7 MB
4D view
SSBD:OMERO
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# 1264
Datast ID FigS1G-E14.5-3
Dataset Kind Image data
Dataset Size 194.1 MB
4D view
SSBD:OMERO
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# 1265
Datast ID FigS1G-E15.5-2
Dataset Kind Image data
Dataset Size 2.5 GB
4D view
SSBD:OMERO
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# 1266
Datast ID FigS1G-E15.5-3
Dataset Kind Image data
Dataset Size 153.6 MB
4D view
SSBD:OMERO
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# 1267
Datast ID FigS1G-E16.5-2
Dataset Kind Image data
Dataset Size 4.2 GB
4D view
SSBD:OMERO
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# 1268
Datast ID FigS1G-E16.5-3
Dataset Kind Image data
Dataset Size 7.4 GB
4D view
SSBD:OMERO
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# 1269
Datast ID FigS3-E14.5-2
Dataset Kind Image data
Dataset Size 5.0 GB
4D view
SSBD:OMERO
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# 1270
Datast ID FigS3-E14.5-3
Dataset Kind Image data
Dataset Size 13.6 GB
4D view
SSBD:OMERO
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# 1271
Datast ID FigS3-E15.5-2
Dataset Kind Image data
Dataset Size 1.8 GB
4D view
SSBD:OMERO
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# 1272
Datast ID FigS3-E15.5-3
Dataset Kind Image data
Dataset Size 6.3 GB
4D view
SSBD:OMERO
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# 1273
Datast ID FigS3-E16.5-2
Dataset Kind Image data
Dataset Size 3.6 GB
4D view
SSBD:OMERO
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# 1274
Datast ID FigS3-E16.5-3-1
Dataset Kind Image data
Dataset Size 2.7 GB
4D view
SSBD:OMERO
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# 1275
Datast ID FigS3-E16.5-3-2
Dataset Kind Image data
Dataset Size 3.2 GB
4D view
SSBD:OMERO
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# 1276
Datast ID FigS4-E14.5
Dataset Kind Image data
Dataset Size 6.2 MB
4D view
SSBD:OMERO
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# 1277
Datast ID FigS4-E18.5
Dataset Kind Image data
Dataset Size 50.3 MB
4D view
SSBD:OMERO
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# 1278
Datast ID FigS4-E15.5
Dataset Kind Image data
Dataset Size 6.1 MB
4D view
SSBD:OMERO
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