Summary of 323-Kang-ZygoteElongate

SSBD:database
SSBD:database URL
Title
Coordinate normalization of Arabidopsis zygote growth.
Description
-
Relase date
2024-11-25
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
2 ( Image datasets: 2, Quantitative data datasets: 0 )
Size of Datasets
2.9 GB ( Image datasets: 2.9 GB, Quantitative data datasets: 0 bytes )

Organism(s)
Arabidopsis thaliana
Gene symbol(s)
NA
Protein name(s)
NA

Datatype
-
Molecular Function (MF)
Biological Process (BP)
zygote elongation
Cellular Component (CC)
plasma membrane, nucleus
Biological Imaging Method
time lapse microscopy, two-photon laser scanning microscopy
T scale
15 minutes per time interval, 60 minutes per time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Zichen Kang, Hikari Matsumoto, Tomonobu Nonoyama, Sakumi Nakagawa, Yukitaka Ishimoto, Satoru Tsugawa, Minako Ueda (2023) Coordinate Normalization of Live-Cell Imaging Data Reveals Growth Dynamics of the Arabidopsis Zygote., Plant & cell physiology

Published in 2023 Mar 20 (Electronic publication in March 20, 2023, midnight )

(Abstract) Polarization of the zygote defines the body axis during plant development. In Arabidopsis (Arabidopsis thaliana), the zygote becomes polarized and elongates in the longitudinal direction, ultimately forming the apical-basal axis of the mature plant. Despite its importance, the mechanism for this elongation remains poorly understood. Based on live-cell imaging of the zygote, we developed new image analysis methods, referred to as coordinate normalization, that appropriately fix and align positions in an image, preventing fluctuation across a temporal sequence of images. Using these methods, we discovered that the zygote elongates only at its apical tip region, similar to tip-growing cells such as pollen tubes and root hairs. We also investigated the spatiotemporal dynamics of the apical tip contour of the zygote and observed that the zygote tip retains its isotropic, hemispherical apical shape during cell elongation. By looking at the elliptical fitting of the contour over time, we further discovered that the apical cell tip becomes thinner at first and then thickens, with a transient increase in growth speed that is followed by the first cell division. We performed the same series of analyses using root hairs and established that the hemispherical tip shape and the changes in growth rate associated with changes in tip size are both specific to the zygote. In summary, the Arabidopsis zygote undergoes directional elongation as a tip-growing cell, but its tip retains an unusual isotropic shape, and the manner of growth changes with the developmental stage.

Contact
Satoru Tsugawa, Minako Ueda , Akita Prefectural University, Tohoku University , Department of Mechanical Engineering , Graduate School of Life Sciences
Contributors
Hikari Matsumoto Zichen Kang


Dataset List of 323-Kang-ZygoteElongate

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
Download BDML
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# 11590
Datast ID Fig1C_zygote_PM
Dataset Kind Image data
Dataset Size 1003.3 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 11591
Dataset Kind Image data
Dataset Size 1.9 GB
4D view
SSBD:OMERO
Download BDML
Download Image data