Summary of 313-Kawano-WormSleep

SSBD:database
SSBD:database URL
Title
Time-lapse images of C. elegans lethargus dynamics by rapid C. elegans motion imaging (Remi) system
Description
-
Relase date
2024-11-25
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
1 ( Image datasets: 1, Quantitative data datasets: 0 )
Size of Datasets
3.6 GB ( Image datasets: 3.6 GB, Quantitative data datasets: 0 bytes )

Organism(s)
Caenorhabditis elegans 
Strain(s)
N2, SLP544, SLP266, SLP808
Gene symbol(s)
NA
Protein name(s)
NA

Datatype
-
Molecular Function (MF)
Biological Process (BP)
sleep
Cellular Component (CC)
Biological Imaging Method
rapid C. elegans motion imaging (Remi) system, time lapse microscopy
T scale
2 seconds per time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Taizo Kawano, Mitsuaki Kashiwagi, Mika Kanuka, Chung-Kuan Chen, Shinnosuke Yasugaki, Sena Hatori, Shinichi Miyazaki, Kaeko Tanaka, Hidetoshi Fujita, Toshiro Nakajima, Masashi Yanagisawa, Yoshimi Nakagawa, Yu Hayashi (2023) ER proteostasis regulators cell-non-autonomously control sleep., Cell reports, Volume 42, Number 3, pp. 112267

Published in 2023 Mar 15 (Electronic publication in March 15, 2023, midnight )

(Abstract) Sleep is regulated by peripheral tissues under fatigue. The molecular pathways in peripheral cells that trigger systemic sleep-related signals, however, are unclear. Here, a forward genetic screen in C. elegans identifies 3 genes that strongly affect sleep amount: sel-1, sel-11, and mars-1. sel-1 and sel-11 encode endoplasmic reticulum (ER)-associated degradation components, whereas mars-1 encodes methionyl-tRNA synthetase. We find that these machineries function in non-neuronal tissues and that the ER unfolded protein response components inositol-requiring enzyme 1 (IRE1)/XBP1 and protein kinase R-like ER kinase (PERK)/eukaryotic initiation factor-2alpha (eIF2alpha)/activating transcription factor-4 (ATF4) participate in non-neuronal sleep regulation, partly by reducing global translation. Neuronal epidermal growth factor receptor (EGFR) signaling is also required. Mouse studies suggest that this mechanism is conserved in mammals. Considering that prolonged wakefulness increases ER proteostasis stress in peripheral tissues, our results suggest that peripheral ER proteostasis factors control sleep homeostasis. Moreover, based on our results, peripheral tissues likely cope with ER stress not only by the well-established cell-autonomous mechanisms but also by promoting the individual's sleep.

Contact
Yu Hayashi , University of Tsukuba , International Institute for Integrative Sleep Medicine (WPI-IIIS)
Contributors
Taizo Kawano


Dataset List of 313-Kawano-WormSleep

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
Download BDML
Download Images
# 11592
Datast ID Worm_lethargus
Dataset Kind Image data
Dataset Size 3.6 GB
4D view
SSBD:OMERO
Download BDML
Download Image data