155 microseconds per frame, 10 millisecond per frame, 311 microseconds per frame, 50 millisecond per frame
Image Acquisition
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Related paper(s)
Kai Lu, Tetsuichi Wazawa, Joe Sakamoto, Cong Quang Vu, Masahiro Nakano, Yasuhiro Kamei, Takeharu Nagai (2022) Intracellular Heat Transfer and Thermal Property Revealed by Kilohertz Temperature Imaging with a Genetically Encoded Nanothermometer., Nano letters, Volume 22, Number 14, pp. 5698-5707
Published in 2022 Jul 27
(Electronic publication in July 6, 2022, midnight )
(Abstract) Despite improved sensitivity of nanothermometers, direct observation of heat transport inside single cells has remained challenging for the lack of high-speed temperature imaging techniques. Here, we identified insufficient temperature resolution under short signal integration time and slow sensor kinetics as two major bottlenecks. To overcome the limitations, we developed B-gTEMP, a nanothermometer based on the tandem fusion of mNeonGreen and tdTomato fluorescent proteins. We visualized the propagation of heat inside intracellular space by tracking the temporal variation of local temperature at a time resolution of 155 mus and a temperature resolution 0.042 degrees C. By comparing the fast in situ temperature dynamics with computer-simulated heat diffusion, we estimated the thermal diffusivity of live HeLa cells. The present thermal diffusivity in cells was about 1/5.3 of that of water and much smaller than the values reported for bulk tissues, which may account for observations of heterogeneous intracellular temperature distributions.