Summary of 118-Iraha-hESCRetina

SSBD:database
SSBD:database URL
Title
Electron microscopy of mature photoreceptors in hESC-Dereived retina rosette-like structure
Description
-
Relase date
2019-11-20
Updated date
-
License
CC BY-NC-SA
Kind
Image data based on Experiment
Number of Datasets
4 ( Image datasets: 4, Quantitative data datasets: 0 )
Size of Datasets
24.6 MB ( Image datasets: 24.6 MB, Quantitative data datasets: 0 bytes )

Organism(s)
H. sapiens

Datatype
tissue morphology
Molecular Function (MF)
Biological Process (BP)
eye photoreceptor cell development, retinal development
Cellular Component (CC)
photoreceptor cell cilium
Biological Imaging Method
-
XYZ Scale
XY: 17 micrometer/pixel, Z: NA, XY: NA, Z: NA
T scale
-

Image Acquisition
Experiment type
Other
Microscope type
BrightfieldMicroscope
Acquisition mode
WideField
Contrast method
Brightfield
Microscope model
Electron microscope
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Satoshi Iraha, Hung-Ya Tu, Suguru Yamasaki, Takahiro Kagawa, Motohito Goto, Riichi Takahashi, Takehito Watanabe, Sunao Sugita, Shigenobu Yonemura, Genshiro A Sunagawa, Take Matsuyama, Momo Fujii, Atsushi Kuwahara, Akiyoshi Kishino, Naoshi Koide, Mototsugu Eiraku, Hidenobu Tanihara, Masayo Takahashi, Michiko Mandai (2018) Establishment of Immunodeficient Retinal Degeneration Model Mice and Functional Maturation of Human ESC-Derived Retinal Sheets after Transplantation., Stem cell reports, Volume 10, Number 3, pp. 1059-1074

Published in 2018 Mar 13 (Electronic publication in March 1, 2018, midnight )

(Abstract) Increasing demand for clinical retinal degeneration therapies featuring human ESC/iPSC-derived retinal tissue and cells warrants proof-of-concept studies. Here, we established two mouse models of end-stage retinal degeneration with immunodeficiency, NOG-rd1-2J and NOG-rd10, and characterized disease progress and immunodeficient status. We also transplanted human ESC-derived retinal sheets into NOG-rd1-2J and confirmed their long-term survival and maturation of the structured graft photoreceptor layer, without rejection or tumorigenesis. We recorded light responses from the host ganglion cells using a multi-electrode array system; this result was consistent with whole-mount immunostaining suggestive of host-graft synapse formation at the responding sites. This study demonstrates an application of our mouse models and provides a proof of concept for the clinical use of human ESC-derived retinal sheets.
(MeSH Terms)

Contact
Michiko Mandai , RIKEN , Center for Biosystems Dynamics Research , Laboratory for Retinal Regeneration
Contributors
Satoshi Iraha, Hung-Ya Tu, Suguru Yamasaki, Takahiro Kagawa, Motohito Goto, Riichi Takahashi, Takehito Watanabe, Sunao Sugita, Shigenobu Yonemura, Genshiro A. Sunagawa, Take Matsuyama, Momo Fujii, Atsushi Kuwahara, Akiyoshi Kishino, Naoshi Koide, Mototsugu Eiraku, Hidenobu Tanihara, Masayo Takahashi, Michiko Mandai


Dataset List of 118-Iraha-hESCRetina

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
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# 4691
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 4692
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
Download BDML
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# 4693
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
Download BDML
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# 4694
Dataset Kind Image data
Dataset Size 15.6 MB
4D view
SSBD:OMERO
Download BDML
Download Image data