Detail of 20130414_1115_04_Graft4th_x2500
Project
Title
Transmission electron microscopy (TEM) image of retinal tissue from Nrl-GFP mouse induced pluripotent stem cells (miPSC) at differentiation day (DD) 17
Description
NA
Release, Updated
2017-10-03,
2018-11-15
2018-11-15
License
CC BY
Kind
Image data
based on Experiment
File Formats
Data size
4.0 MB
Gene symbols
Nrl
Protein tags
GFP
Datatype
tissue structure
Molecular Function (MF)
Biological Process (BP)
-
Cellular Component (CC)
cell
(
GO:0005623
)
Biological Imaging Method
XYZ Scale
XY: 0.00907 micrometer/pixel, Z: NA
T scale
-
Image Acquisition
Experiment type
Other
Microscope type
Other
Acquisition mode
Other
Contrast method
Other
Microscope model
JEOL JEM 1010 or JEM 1400
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Assawachananont et al. (2014) Stem Cell Reports, 2(5): 662-674.
Related paper(s)
Juthaporn Assawachananont, Michiko Mandai, Satoshi Okamoto, Chikako Yamada, Mototsugu Eiraku, Shigenobu Yonemura, Yoshiki Sasai, Masayo Takahashi (2014) Transplantation of embryonic and induced pluripotent stem cell-derived 3D retinal sheets into retinal degenerative mice., Stem cell reports, Volume 2, Number 5, pp. 662-74
Published in 2014 May 6 (Electronic publication in April 24, 2014, midnight )
- doi: 10.1016/j.stemcr.2014.03.011
-
pmid: 24936453
(Abstract) In this article, we show that mouse embryonic stem cell- or induced pluripotent stem cell-derived 3D retinal tissue developed a structured outer nuclear layer (ONL) with complete inner and outer segments even in an advanced retinal degeneration model (rd1) that lacked ONL. We also observed host-graft synaptic connections by immunohistochemistry. This study provides a "proof of concept" for retinal sheet transplantation therapy for advanced retinal degenerative diseases.(MeSH Terms)
- Animals
- Cell Culture Techniques
- Collagen/chemistry
- Drug Combinations
- Embryonic Stem Cells/cytology
- Embryonic Stem Cells/transplantation[major]
- Immunohistochemistry
- Induced Pluripotent Stem Cells/cytology
- Induced Pluripotent Stem Cells/transplantation[major]
- Laminin/chemistry
- Mice
- Mice, Inbred C3H
- Mice, Inbred C57BL
- Mitochondria/metabolism
- Mitochondria/pathology
- Proteoglycans/chemistry
- Receptors, Retinoic Acid/antagonists & inhibitors
- Retina/metabolism
- Retina/pathology[major]
- Retinal Degeneration/therapy[major]
- Retinal Degeneration/veterinary
- Transplantation, Homologous
Contact
Michiko Mandai
, RIKEN
, Center for Developmental Biology
, Laboratory for Retinal Regeneration
Contributors
Juthaporn Assawachananont, Michiko Mandai, Satoshi Okamoto, Chikako Yamada, Mototsugu Eiraku, Shigenobu Yonemura, Yoshiki Sasai, Masayo Takahashi
OMERO Dataset
OMERO Project
Source