Detail of FigS11A_R_R04



Project
Title
Two-photon images of Thy1-YFP-H mouse hemi-brain (P21) cleared with SeeDB37
Description
NA
Release, Updated
2017-10-03,
2017-11-15
License
CC BY
Kind
Image data based on Experiment
File Formats
Data size
127.8 MB

Organism
M. musculus ( NCBI:txid10090 )
Strain(s)
-
Cell Line
-
Gene symbols
Thy1
Protein tags
YFP

Datatype
neuron structure
Molecular Function (MF)
Biological Process (BP)
-
Cellular Component (CC)
-
Biological Imaging Method
XYZ Scale
XY: 1.382 micrometer/pixel, Z: 5.0 micrometer/slice
T scale
-

Image Acquisition
Experiment type
Other
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
Olympus FV1000MPE (two-photon)
Detector model
GaAsP
Objective model
Olympus XLSLPLN25XSVMP 25x/0.9
Filter set
-

Summary of Methods
See details in Ke et al. (2013) Nature Neuroscience, 16: 1154-1161.
Related paper(s)

Meng-Tsen Ke, Satoshi Fujimoto, Takeshi Imai (2013) SeeDB: a simple and morphology-preserving optical clearing agent for neuronal circuit reconstruction., Nature neuroscience, Volume 16, Number 8, pp. 1154-61

Published in 2013 Aug (Electronic publication in June 23, 2013, midnight )

(Abstract) We report a water-based optical clearing agent, SeeDB, which clears fixed brain samples in a few days without quenching many types of fluorescent dyes, including fluorescent proteins and lipophilic neuronal tracers. Our method maintained a constant sample volume during the clearing procedure, an important factor for keeping cellular morphology intact, and facilitated the quantitative reconstruction of neuronal circuits. Combined with two-photon microscopy and an optimized objective lens, we were able to image the mouse brain from the dorsal to the ventral side. We used SeeDB to describe the near-complete wiring diagram of sister mitral cells associated with a common glomerulus in the mouse olfactory bulb. We found the diversity of dendrite wiring patterns among sister mitral cells, and our results provide an anatomical basis for non-redundant odor coding by these neurons. Our simple and efficient method is useful for imaging intact morphological architecture at large scales in both the adult and developing brains.
(MeSH Terms)

Contact
Takeshi Imai , RIKEN , Center for Developmental Biology , Laboratory for Sensory Circuit Formation
Contributors
Meng-Tsen Ke, Satoshi Fujimoto, Takeshi Imai

OMERO Dataset
OMERO Project
External Link
Source