Detail of Figure2abc_epithelium_morphology

Images of the epidermal model Acidic vesicles in granular cells labelled with Hoechst 33258, CTG, and LTR.
Images of the epidermal model acidic vesicles in granular cells labelled with Hoechst 33258, CTG, and LTR.
Release, Updated
Image data
File Formats
Data size
528.6 MB

Homo sapiens ( NCBITaxon:9606 )
Cell Line
Hoechst 33258, CTG, LTR

Molecular Function (MF)
Biological Process (BP)
keratinocyte activation ( GO:0032980 )
Cellular Component (CC)
nucleus ( GO:0005634 )
Biological Imaging Method
two-photon laser scanning microscopy ( Fbbi:00000254 )
X scale
0.1563 micrometer/pixel
Y scale
0.1563 micrometer/pixel
Z scale
0.6 micrometer/slice
T scale

Image Acquisition
Experiment type
Microscope type
Acquisition mode
Contrast method
Microscope model
Detector model
Objective model
Filter set

Summary of Methods
See details in Ipponjima S, et. al. (2020) Sci Rep., 10(1):5515.
Related paper(s)

Sari Ipponjima, Yuki Umino, Masaharu Nagayama, Mitsuhiro Denda (2020) Live imaging of alterations in cellular morphology and organelles during cornification using an epidermal equivalent model., Scientific reports, Volume 10, Number 1, pp. 5515

Published in 2020 Mar 26 (Electronic publication in March 26, 2020, midnight )

(Abstract) The stratum corneum plays a crucial role in epidermal barrier function. Various changes occur in granular cells at the uppermost stratum granulosum during cornification. To understand the temporal details of this process, we visualized the cell shape and organelles of cornifying keratinocytes in a living human epidermal equivalent model. Three-dimensional time-lapse imaging with a two-photon microscope revealed that the granular cells did not simply flatten but first temporarily expanded in thickness just before flattening during cornification. Moreover, before expansion, intracellular vesicles abruptly stopped moving, and mitochondria were depolarized. When mitochondrial morphology and quantity were assessed, granular cells with fewer, mostly punctate mitochondria tended to transition to corneocytes. Several minutes after flattening, DNA leakage from the nucleus was visualized. We also observed extension of the cell-flattening time induced by the suppression of filaggrin expression. Overall, we successfully visualized the time-course of cornification, which describes temporal relationships between alterations in the transition from granular cells to corneocytes.
(MeSH Terms)

Sari Ipponjima , Hokkaido University , Research Center of Mathematics for Social Creativity, Research Institute for Electronic Science

OMERO Dataset
OMERO Project