Detail of Fig1D_myogenicCell_5dpi

live imaging of regenerating muscles expressing EKAREV and tdKeima at 5 days post-injury (dpi).
live imaging of regenerating muscles expressing EKAREV and tdKeima at 5 days post-injury (dpi).
Release, Updated
Image data
File Formats
Data size
317.1 MB

Mus musculus ( NCBITaxon:10090 )
Cell Line
EKAREV, tdKeima

Molecular Function (MF)
extracellular signal-regulated kinase activity ( GO:0004707 )
Biological Process (BP)
cell migration ( GO:0016477 )
Cellular Component (CC)
Biological Imaging Method
two-photon laser scanning microscopy ( Fbbi:00000254 )
X scale
1.59 micrometer/pixel
Y scale
1.59 micrometer/pixel
Z scale
2 micrometer/slice
T scale
1 sec per time interval

Image Acquisition
Experiment type
Microscope type
Acquisition mode
Contrast method
Microscope model
Detector model
Objective model
Filter set

Summary of Methods
See details in Konagaya Y, et. al. (2020) Cell Cycle., 19(22):3167-3181.
Related paper(s)

Yumi Konagaya, Kanako Takakura, Maina Sogabe, Anjali Bisaria, Chad Liu, Tobias Meyer, Atsuko Sehara-Fujisawa, Michiyuki Matsuda, Kenta Terai (2020) Intravital imaging reveals cell cycle-dependent myogenic cell migration during muscle regeneration., Cell cycle (Georgetown, Tex.), Volume 19, Number 22, pp. 3167-3181

Published in 2020 Nov (Electronic publication in Nov. 1, 2020, midnight )

(Abstract) During muscle regeneration, extracellular signal-regulated kinase (ERK) promotes both proliferation and migration. However, the relationship between proliferation and migration is poorly understood in this context. To elucidate this complex relationship on a physiological level, we established an intravital imaging system for measuring ERK activity, migration speed, and cell-cycle phases in mouse muscle satellite cell-derived myogenic cells. We found that in vivo, ERK is maximally activated in myogenic cells two days after injury, and this is then followed by increases in cell number and motility. With limited effects of ERK activity on migration on an acute timescale, we hypothesized that ERK increases migration speed in the later phase by promoting cell-cycle progression. Our cell-cycle analysis further revealed that in myogenic cells, ERK activity is critical for G1/S transition, and cells migrate more rapidly in S/G2 phase 3 days after injury. Finally, migration speed of myogenic cells was suppressed after CDK1/2-but not CDK1-inhibitor treatment, demonstrating a critical role of CDK2 in myogenic cell migration. Overall, our study demonstrates that in myogenic cells, the ERK-CDK2 axis promotes not only G1/S transition but also migration, thus providing a novel mechanism for efficient muscle regeneration.
(MeSH Terms)

Kenta Terai , Graduate School of Medicine, Kyoto University , Department of Pathology and Biology of Diseases , Department of Pathology and Biology of Diseases
Yumi Konagaya

OMERO Dataset
OMERO Project