Detail of Fig1D_DMRE_110120-1

Project
Title
Calcium response (0.2 s interval) and shape change (1.6 s interval) in oocyte during fertilization in wild-type C. elegans related to Fig. 1D in Takayama and Onami (2016).
Description
NA
Release, Updated
2016-10-03,
2020-02-03
License
CC BY-NC-SA
Kind
Image data based on Experiment related Quantitative data ; Fig1D_DMRE_110120-1

Organism
C. elegans ( NCBITaxon:6239 )
Strain(s)
-
Cell Line
-

Datatype
oocyte shape dynamics
Molecular Function (MF)
Biological Process (BP)
egg activation ( GO:0007343 )
Cellular Component (CC)
cytoplasm ( GO:0005737 )
Biological Imaging Method
Image Acquisition
-
XYZ Scale
XY: 0.417 micrometer/pixel, Z: 0 micrometer/slice
T scale
1.6 second for each time interval

Summary of Methods
See details in Takayama and Onami. (2016) Cell Reports, 15(3), 625-637
Related paper(s)

Jun Takayama, Shuichi Onami (2016) The Sperm TRP-3 Channel Mediates the Onset of a Ca(2+) Wave in the Fertilized C. elegans Oocyte., Cell reports, Volume 15, Number 3, pp. 625-637

Published in 2016 Apr 19 (Electronic publication in April 7, 2016, midnight )

(Abstract) Sperm induce Ca(2+) waves in the fertilized egg by introducing soluble factors or by surface interactions, which activate egg Ca(2+) channels. Involvement of sperm Ca(2+) channels is predicted by the conduit model; however, this model has not been validated. In Caenorhabditis elegans, the sperm-specific TRP family Ca(2+) channel TRP-3 mediates sperm-oocyte fusion. Here, using high-speed in vivo imaging and image analyses, we show that sperm induce an immediate local Ca(2+) rise followed by a Ca(2+) wave in fertilized C. elegans oocytes. Oocytes fertilized by rare trp-3 escaper sperm showed a lack of local rise and a delay in onset of the Ca(2+) wave. Sperm Ca(2+) imaging suggests that the local rise is not due to the bolus introduction of stored Ca(2+). These results suggest that, along with its primary function in sperm-oocyte fusion, TRP-3 induces Ca(2+) waves in fertilized oocytes, consistent with the conduit model.
(MeSH Terms)

Contact
Shuichi Onami , RIKEN , Quantitative Biology Center , Laboratory for Developmental Dynamics
Contributors
Jun Takayama, Shuichi Onami

OMERO Dataset
OMERO Project
Source