Detail of Fig1D_DMRE_110120-1

Calcium response (0.2 s interval) and shape change (1.6 s interval) in oocyte during fertilization in wild-type C. elegans related to Fig. 1D in Takayama and Onami (2016).
Release, Updated
Image data based on Experiment related Quantitative data ; Fig1D_DMRE_110120-1
File Formats
Data size
84.5 MB

C. elegans ( NCBITaxon:6239 )
Cell Line

oocyte shape dynamics
Molecular Function (MF)
Biological Process (BP)
egg activation ( GO:0007343 )
Cellular Component (CC)
cytoplasm ( GO:0005737 )
Biological Imaging Method
XYZ Scale
XY: 0.417 micrometer/pixel, Z: 0 micrometer/slice
T scale
1.6 second for each time interval

Image Acquisition
Experiment type
Microscope type
Acquisition mode
Contrast method
Microscope model
Detector model
Objective model
Filter set

Summary of Methods
See details in Takayama and Onami. (2016) Cell Reports, 15(3), 625-637
Related paper(s)

Jun Takayama, Shuichi Onami (2016) The Sperm TRP-3 Channel Mediates the Onset of a Ca(2+) Wave in the Fertilized C. elegans Oocyte., Cell reports, Volume 15, Number 3, pp. 625-637

Published in 2016 Apr 19 (Electronic publication in April 7, 2016, midnight )

(Abstract) Sperm induce Ca(2+) waves in the fertilized egg by introducing soluble factors or by surface interactions, which activate egg Ca(2+) channels. Involvement of sperm Ca(2+) channels is predicted by the conduit model; however, this model has not been validated. In Caenorhabditis elegans, the sperm-specific TRP family Ca(2+) channel TRP-3 mediates sperm-oocyte fusion. Here, using high-speed in vivo imaging and image analyses, we show that sperm induce an immediate local Ca(2+) rise followed by a Ca(2+) wave in fertilized C. elegans oocytes. Oocytes fertilized by rare trp-3 escaper sperm showed a lack of local rise and a delay in onset of the Ca(2+) wave. Sperm Ca(2+) imaging suggests that the local rise is not due to the bolus introduction of stored Ca(2+). These results suggest that, along with its primary function in sperm-oocyte fusion, TRP-3 induces Ca(2+) waves in fertilized oocytes, consistent with the conduit model.
(MeSH Terms)

Shuichi Onami , RIKEN , Quantitative Biology Center , Laboratory for Developmental Dynamics
Jun Takayama, Shuichi Onami

OMERO Dataset
OMERO Project