Detail of Figure4_Prox1_E10.5
Project
Title
Immunohistochemistry image of Prox1 expression at mouse E10.5 in the pharyngeal core mesoderm
Description
NA
Release, Updated
2019-11-20
License
CC BY
Kind
Image data
based on Experiment
File Formats
Data size
19.9 MB
Gene symbols
Prox1
Datatype
Immunostaining
Molecular Function (MF)
Biological Process (BP)
lymphatic vascular process in circulatory system
(
GO:1990183
)
lymphatic endothelial cell differentiation
(
GO:0060836
)
Cellular Component (CC)
-
Biological Imaging Method
XYZ Scale
XY: 264.58 micrometer/pixel, Z: NA
T scale
-
Image Acquisition
Experiment type
Immunohistochemistry
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
Nikon C2
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Maruyama et al. (2019) Dev Biol, 452(2): 134-143.
Related paper(s)
Kazuaki Maruyama, Sachiko Miyagawa-Tomita, Kaoru Mizukami, Fumio Matsuzaki, Hiroki Kurihara (2019) Isl1-expressing non-venous cell lineage contributes to cardiac lymphatic vessel development., Developmental biology, Volume 452, Number 2, pp. 134-143
Published in 2019 Aug 15 (Electronic publication in May 18, 2019, midnight )
- doi: 10.1016/j.ydbio.2019.05.002
-
pmid: 31112709
(Abstract) The origin of the mammalian lymphatic vasculature has been studied for more than a century; however, details regarding organ-specific lymphatic development remain unknown. A recent study reported that cardiac lymphatic endothelial cells (LECs) stem from venous and non-venous origins in mice. Here, we identified Isl1-expressing progenitors as a potential non-venous origin of cardiac LECs. Genetic lineage tracing with Isl1-Cre reporter mice suggested a possible contribution from the Isl1-expressing pharyngeal mesoderm constituting the second heart field to lymphatic vessels around the cardiac outflow tract as well as to those in the facial skin and the lymph sac. Isl1(+) lineage-specific deletion of Prox1 resulted in disrupted LYVE1(+) vessel structures, indicating a Prox1-dependent mechanism in this contribution. Tracing back to earlier embryonic stages revealed the presence of VEGFR3(+) and/or Prox1(+) cells that overlapped with the Isl1(+) pharyngeal core mesoderm. These data may provide insights into the developmental basis of heart diseases involving lymphatic vasculature and improve our understanding of organ-based lymphangiogenesis.(MeSH Terms)
- Animals
- Cell Lineage[major]
- Endothelial Cells/metabolism
- Heart/embryology[major]
- Homeodomain Proteins/metabolism
- LIM-Homeodomain Proteins/metabolism[major]
- Lymphangiogenesis[major]
- Lymphatic Vessels/cytology[major]
- Lymphatic Vessels/embryology[major]
- Mesoderm/embryology
- Mesoderm/metabolism
- Mice
- Pharynx/cytology
- Stem Cells/metabolism
- Transcription Factors/metabolism[major]
- Tumor Suppressor Proteins/metabolism
- Vascular Endothelial Growth Factor Receptor-3/metabolism
Contact
Hiroki Kurihara
, The University of Tokyo
, Graduate School of Medicine
, Department of Physiological Chemistry and Metabolism
Contributors
Kazuaki Maruyama, Sachiko Miyagawa-Tomita, Kaoru Mizukami, Fumio Matsuzaki, Hiroki Kurihara
OMERO Dataset
OMERO Project
Source