Detail of Figure4_Prox1_E9.5



Project
Title
Immunohistochemistry image of Prox1 expression at mouse E9.5 in the pharyngeal core mesoderm
Description
NA
Release, Updated
2019-11-20
License
CC BY
Kind
Image data based on Experiment
File Formats
Data size
27.5 MB

Organism
M. musculus ( NCBI:txid10090 )
Strain(s)
-
Cell Line
-
Gene symbols
Prox1

Datatype
Immunostaining
Molecular Function (MF)
Biological Process (BP)
lymphatic vascular process in circulatory system ( GO:1990183 ) lymphatic endothelial cell differentiation ( GO:0060836 )
Cellular Component (CC)
-
Biological Imaging Method
XYZ Scale
XY: 264.58 micrometer/pixel, Z: NA
T scale
-

Image Acquisition
Experiment type
Immunohistochemistry
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
Nikon C2
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Maruyama et al. (2019) Dev Biol, 452(2): 134-143.
Related paper(s)

Kazuaki Maruyama, Sachiko Miyagawa-Tomita, Kaoru Mizukami, Fumio Matsuzaki, Hiroki Kurihara (2019) Isl1-expressing non-venous cell lineage contributes to cardiac lymphatic vessel development., Developmental biology, Volume 452, Number 2, pp. 134-143

Published in 2019 Aug 15 (Electronic publication in May 18, 2019, midnight )

(Abstract) The origin of the mammalian lymphatic vasculature has been studied for more than a century; however, details regarding organ-specific lymphatic development remain unknown. A recent study reported that cardiac lymphatic endothelial cells (LECs) stem from venous and non-venous origins in mice. Here, we identified Isl1-expressing progenitors as a potential non-venous origin of cardiac LECs. Genetic lineage tracing with Isl1-Cre reporter mice suggested a possible contribution from the Isl1-expressing pharyngeal mesoderm constituting the second heart field to lymphatic vessels around the cardiac outflow tract as well as to those in the facial skin and the lymph sac. Isl1(+) lineage-specific deletion of Prox1 resulted in disrupted LYVE1(+) vessel structures, indicating a Prox1-dependent mechanism in this contribution. Tracing back to earlier embryonic stages revealed the presence of VEGFR3(+) and/or Prox1(+) cells that overlapped with the Isl1(+) pharyngeal core mesoderm. These data may provide insights into the developmental basis of heart diseases involving lymphatic vasculature and improve our understanding of organ-based lymphangiogenesis.
(MeSH Terms)

Contact
Hiroki Kurihara , The University of Tokyo , Graduate School of Medicine , Department of Physiological Chemistry and Metabolism
Contributors
Kazuaki Maruyama, Sachiko Miyagawa-Tomita, Kaoru Mizukami, Fumio Matsuzaki, Hiroki Kurihara

OMERO Dataset
OMERO Project
Source