Detail of Fig2_FigS2_TERT_2
Project
Title
Time-lapse images of HeLa cells with or without expressing the TERT constructs during apoptosis
Description
Time-lapse images of HeLa cells with or without expressing the TERT (Telomerase reverse transcriptase) constructs during apoptosis induced by oxidative stress. This dataset is composed of 16 series of dataset; series1-series16, each of which is 3-day long time-lapse image of 7 x 7 tiling data. Channel2; bright field, Channel4; SYTOX Orange, Channel5; merge. The 16 series consist of 4 genotypes and 4 experimental conditions. Genotypes: Series1-Series4; without transfection, Series5-Series8; TERT transfection, Series9-Series12; mutant TERT (R3ER6E) transfection, Series13-Series16; mutant TERT (Y707F) transfection. Experimental conditions: Series1, 5, 9, 13; SYTOX Orange was used as an apotosis marker, Series2, 6, 10, 14; YO-PRO-1 was used as an apotosis marker, Series3, 7, 11, 15; SYTOX Orange imaging with treatment of Z-VAD-fmk, Series 4, 8, 12, 16; SYTOX Orange imaging with treatment of GSK'872.
Release, Updated
2024-12-24
License
CC BY
Kind
Image data
File Formats
.nd2
Data size
564.5 GB
Datatype
-
Molecular Function (MF)
telomerase activity
Biological Process (BP)
regulation of apoptotic process
Cellular Component (CC)
mitochondrion
nucleus
Biological Imaging Method
X scale
0.324 micrometer
Y scale
0.324 micrometer
Z scale
-
T scale
1 hour
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Ebata H, Shima T, Iizuka R, Uemura S. Accumulation of TERT in mitochondria exerts two opposing effects on apoptosis. FEBS Open Bio. 2023, Sep;13(9) :1667-1682.
Related paper(s)
Hiroshi Ebata, Tomohiro Shima, Ryo Iizuka, Sotaro Uemura (2023) Accumulation of TERT in mitochondria exerts two opposing effects on apoptosis., FEBS open bio
Published in 2023 Jul 31 (Electronic publication in July 31, 2023, midnight )
- doi: 10.1002/2211-5463.13682
-
pmid: 37525387
(Abstract) Telomerase reverse transcriptase (TERT) is a protein that catalyzes the reverse transcription of telomere elongation. TERT is also expected to play a noncanonical role beyond telomere lengthening since it localizes not only in the nucleus but also in mitochondria, where telomeres do not exist. Several studies have reported that mitochondrial TERT regulates apoptosis induced by oxidative stress. However, there is still some controversy as to whether mitochondrial TERT promotes or inhibits apoptosis, mainly due to the lack of information on changes in TERT distribution in individual cells over time. Here, we simultaneously detected apoptosis and TERT localization after oxidative stress in individual HeLa cells by live-cell tracking. Single-cell tracking revealed that the stress-induced accumulation of TERT in mitochondria caused apoptosis, but that accumulation increased over time until cell death. The results suggest a new model in which mitochondrial TERT has two opposing effects at different stages of apoptosis: it predetermines apoptosis at the first stage of cell-fate determination, but also delays apoptosis at the second stage. As such, our data support a model that integrates the two opposing hypotheses on mitochondrial TERT's effect on apoptosis. Furthermore, detailed statistical analysis of TERT mutations, which have been predicted to inhibit TERT transport to mitochondria, revealed that these mutations suppress apoptosis independent of mitochondrial localization of TERT. Together, these results imply that the non-canonical functions of TERT affect a wide range of mitochondria-dependent and mitochondria-independent apoptosis pathways.
Contact
Tomohiro Shima, Sotaro Uemura
, The University of Tokyo, The University of Tokyo
, Department of Biological Sciences, Graduate School of Science, Department of Biological Sciences, Graduate School of Science
, Department of Biological Sciences, Graduate School of Science, Department of Biological Sciences, Graduate School of Science
Contributors
Hiroshi Ebata
OMERO Project
Source