Summary of 43-Takai-SubcellStructONL

SSBD:database
SSBD:database URL
Title
-
Description
-
Relase date
2017-10-03
Updated date
2018-11-15
License
CC BY-NC-SA
Kind
Image data, Quantitative data based on Experiment
Number of Datasets
4 ( Image datasets: 2, Quantitative data datasets: 2 )
Size of Datasets
90.6 MB ( Image datasets: 90.0 MB, Quantitative data datasets: 620.8 KB )

Organism(s)
C. l. familiaris
Strain(s)
MDCK II
Reporter(s)
LAMP1-ONL, ONL-PTS1

Datatype
lysosomes dynamics, peroxisomes dynamics
Molecular Function (MF)
Biological Process (BP)
cellular protein localization
Cellular Component (CC)
-
Biological Imaging Method
-
XYZ Scale
XY: 0.250 micrometer/pixel, Z: NA
T scale
7.8 second for each time interval, 8.8 second for each time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Akira Takai, Masahiro Nakano, Kenta Saito, Remi Haruno, Tomonobu M Watanabe, Tatsuya Ohyanagi, Takashi Jin, Yasushi Okada, Takeharu Nagai (2015) Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging., Proceedings of the National Academy of Sciences of the United States of America, Volume 112, Number 14, pp. 4352-6

Published in 2015 Apr 7 (Electronic publication in March 23, 2015, midnight )

(Abstract) Fluorescence live imaging has become an essential methodology in modern cell biology. However, fluorescence requires excitation light, which can sometimes cause potential problems, such as autofluorescence, phototoxicity, and photobleaching. Furthermore, combined with recent optogenetic tools, the light illumination can trigger their unintended activation. Because luminescence imaging does not require excitation light, it is a good candidate as an alternative imaging modality to circumvent these problems. The application of luminescence imaging, however, has been limited by the two drawbacks of existing luminescent protein probes, such as luciferases: namely, low brightness and poor color variants. Here, we report the development of bright cyan and orange luminescent proteins by extending our previous development of the bright yellowish-green luminescent protein Nano-lantern. The color change and the enhancement of brightness were both achieved by bioluminescence resonance energy transfer (BRET) from enhanced Renilla luciferase to a fluorescent protein. The brightness of these cyan and orange Nano-lanterns was approximately 20 times brighter than wild-type Renilla luciferase, which allowed us to perform multicolor live imaging of intracellular submicron structures. The rapid dynamics of endosomes and peroxisomes were visualized at around 1-s temporal resolution, and the slow dynamics of focal adhesions were continuously imaged for longer than a few hours without photobleaching or photodamage. In addition, we extended the application of these multicolor Nano-lanterns to simultaneous monitoring of multiple gene expression or Ca(2+) dynamics in different cellular compartments in a single cell.
(MeSH Terms)

Contact
Yasushi Okada , RIKEN , Quantitative Biology Center , Laboratory for Cell Polarity Regulation
Contributors
Akira Takai, Masahiro Nakano, Kenta Saito, Remi Haruno, Tomonobu M. Watanabe, Tatsuya Ohyanagi, Takashi Jin, Yasushi Okada, Takeharu Nagai


Dataset List of 43-Takai-SubcellStructONL

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
Download BDML
Download Images
# 1234
Datast ID LAMP1-ONL
Dataset Kind Image data
Dataset Size 45.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 1235
Datast ID LAMP1-ONL
Dataset Kind Quantitative data
Dataset Size 302.8 KB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 1236
Datast ID ONL-PTS1
Dataset Kind Image data
Dataset Size 45.0 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 1237
Datast ID ONL-PTS1
Dataset Kind Quantitative data
Dataset Size 318.0 KB
4D view
SSBD:OMERO
Download BDML
Download Image data