Summary of 4-Keller-FlyEmbryo

SSBD:database
SSBD:database URL
Title
-
Description
-
Relase date
2013-10-03
Updated date
2019-04-24
License
CC BY-NC-SA
Kind
Quantitative data based on Experiment
Number of Datasets
2 ( Image datasets: 0, Quantitative data datasets: 2 )
Size of Datasets
4.0 GB ( Image datasets: 0 bytes, Quantitative data datasets: 4.0 GB )

Organism(s)
D. melanogaster
Gene symbol(s)
NA
Protein name(s)
NA

Datatype
nuclear positions
Molecular Function (MF)
Biological Process (BP)
embryo development
Cellular Component (CC)
nucleus
Biological Imaging Method
-
XYZ Scale
XY: 0.37 micrometer/pixel, Z: 0.37micrometer/frame
T scale
3.0 minute for each time interval

Image Acquisition
Experiment type
TimeLapse
Microscope type
ConfocalMicroscope
Acquisition mode
Other
Contrast method
Other
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Philipp J Keller, Annette D Schmidt, Anthony Santella, Khaled Khairy, Zhirong Bao, Joachim Wittbrodt, Ernst H K Stelzer (2010) Fast, high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopy., Nature methods, Volume 7, Number 8, pp. 637-42

Published in 2010 Aug (Electronic publication in July 4, 2010, midnight )

(Abstract) Recording light-microscopy images of large, nontransparent specimens, such as developing multicellular organisms, is complicated by decreased contrast resulting from light scattering. Early zebrafish development can be captured by standard light-sheet microscopy, but new imaging strategies are required to obtain high-quality data of late development or of less transparent organisms. We combined digital scanned laser light-sheet fluorescence microscopy with incoherent structured-illumination microscopy (DSLM-SI) and created structured-illumination patterns with continuously adjustable frequencies. Our method discriminates the specimen-related scattered background from signal fluorescence, thereby removing out-of-focus light and optimizing the contrast of in-focus structures. DSLM-SI provides rapid control of the illumination pattern, exceptional imaging quality and high imaging speeds. We performed long-term imaging of zebrafish development for 58 h and fast multiple-view imaging of early Drosophila melanogaster development. We reconstructed cell positions over time from the Drosophila DSLM-SI data and created a fly digital embryo.
(MeSH Terms)

Contact
Philipp J. Keller , Molecular Biology Laboratory , Cell Biology and Biophysics Unit
Contributors
Philipp J Keller, Annette D Schmidt, Anthony Santella, Khaled Khairy, Zhirong Bao, Joachim Wittbrodt, Ernst H K Stelzer


Dataset List of 4-Keller-FlyEmbryo

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
Download BDML
Download Images
# 475
Dataset Kind Quantitative data
Dataset Size 885.7 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 476
Dataset Kind Quantitative data
Dataset Size 363.8 MB
4D view
SSBD:OMERO
Download BDML
Download Image data