Summary of 32-Matsuda-SigDyn

SSBD:database
SSBD:database URL
Title
-
Description
-
Relase date
2017-10-03
Updated date
2018-11-15
License
CC BY-NC-SA
Kind
Image data based on Experiment
Number of Datasets
2 ( Image datasets: 2, Quantitative data datasets: 0 )
Size of Datasets
164.6 MB ( Image datasets: 164.6 MB, Quantitative data datasets: 0 bytes )

Organism(s)
C. griseus
Strain(s)
CHO
Gene symbol(s)
NA
Protein name(s)
Delta, tTS
Protein tag(s)
GFP, mCherry

Datatype
cell dynamics
Molecular Function (MF)
Biological Process (BP)
-
Cellular Component (CC)
-
Biological Imaging Method
-
XYZ Scale
XY: 0.160 micrometer/pixel, Z: NA, XY: 1.0 micrometer/pixel, Z: NA
T scale
0.5 hour for each time interval, 1 hour for each time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Mitsuhiro Matsuda, Makito Koga, Knut Woltjen, Eisuke Nishida, Miki Ebisuya (2015) Synthetic lateral inhibition governs cell-type bifurcation with robust ratios., Nature communications, Volume 6, pp. 6195

Published in 2015 Feb 5 (Electronic publication in Feb. 5, 2015, midnight )

(Abstract) Cell-type diversity in multicellular organisms is created through a series of binary cell fate decisions. Lateral inhibition controlled by Delta-Notch signalling is the core mechanism for the choice of alternative cell types by homogeneous neighbouring cells. Here, we show that cells engineered with a Delta-Notch-dependent lateral inhibition circuit spontaneously bifurcate into Delta-positive and Notch-active cell populations. The synthetic lateral inhibition circuit comprises transcriptional repression of Delta and intracellular feedback of Lunatic fringe (Lfng). The Lfng-feedback subcircuit, even alone, causes the autonomous cell-type bifurcation. Furthermore, the ratio of two cell populations bifurcated by lateral inhibition is reproducible and robust against perturbation. The cell-type ratio is adjustable by the architecture of the lateral inhibition circuit as well as the degree of cell-cell attachment. Thus, the minimum lateral inhibition mechanism between adjacent cells not only serves as a binary cell-type switch of individual cells but also governs the cell-type ratio at the cell-population level.
(MeSH Terms)

Contact
Miki Ebisuya , RIKEN , Quantitative Biology Center , Laboratory for Reconstitutive Developmental Biology
Contributors
Mitsuhiro Matsuda, Makito Koga, Knut Woltjen, Eisuke Nishida, Miki Ebisuya


Dataset List of 32-Matsuda-SigDyn

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
Download BDML
Download Images
# 974
Datast ID Fig2-Merge
Dataset Kind Image data
Dataset Size 98.7 MB
4D view
SSBD:OMERO
Download BDML
Download Image data

# 975
Datast ID Fig5-PH
Dataset Kind Image data
Dataset Size 65.9 MB
4D view
SSBD:OMERO
Download BDML
Download Image data