Summary of 295-Katoh-CiliumDyn

SSBD:database
SSBD:database URL
Title
Images of immotile cilia dynamics in wild-type mouse embryo or iv/iv mutant embryo
Description
-
Relase date
2023-07-20
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
18 ( Image datasets: 18, Quantitative data datasets: 0 )
Size of Datasets
19.1 GB ( Image datasets: 19.1 GB, Quantitative data datasets: 0 bytes )

Organism(s)
Mus musculus

Datatype
-
Molecular Function (MF)
Biological Process (BP)
calcium-mediated signaling, determination of left/right symmetry, detection of nodal flow
Cellular Component (CC)
non-motile cilium
Biological Imaging Method
time lapse microscopy, immunostaining, highly inclined and laminated optical sheet (HILO) microscopy, Spinning disk confocal microscopy, Spinning disk confocal microscope with custom-built optical pathway, AiryScan, Optical-tweezers, Spinning disk confocal microscopy with deconvolution, Whole-Cell FRAP (FRAPとは異なります。本論文で初めて報告した新規顕微鏡法です), STED, Calcium imaging
X scale
0.43 micrometer/pixel, 0.215 micrometer/pixel, 0.36 micrometer/pixel, 0.13 micrometer/pixel, 0.0458394 micrometer/pixel, 0.022727 micrometer/pixel
Y scale
0.43 micrometer/pixel, 0.215 micrometer/pixel, 0.36 micrometer/pixel, 0.13 micrometer/pixel, 0.0458394 micrometer/pixel, 0.022727 micrometer/pixel
Z scale
0.2180928 micrometer/slice, 0.1001360 micrometer/slice, 0.20 micrometer/pixel, 1000 micrometer/slice, NA
T scale
29.12 msec per time interval, 2 min per time interval, 100 msec per time interval, 1.122 sec per time interval, 6.550 sec per time interval, 0.292 sec per time interval, NA

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Takanobu A Katoh, Toshihiro Omori, Katsutoshi Mizuno, Xiaorei Sai, Katsura Minegishi, Yayoi Ikawa, Hiromi Nishimura, Takeshi Itabashi, Eriko Kajikawa, Sylvain Hiver, Atsuko H Iwane, Takuji Ishikawa, Yasushi Okada, Takayuki Nishizaka, Hiroshi Hamada (2023) Immotile cilia mechanically sense the direction of fluid flow for left-right determination., Science (New York, N.Y.), Volume 379, Number 6627, pp. 66-71

Published in 2023 Jan 6 (Electronic publication in Jan. 5, 2023, midnight )

(Abstract) Immotile cilia at the ventral node of mouse embryos are required for sensing leftward fluid flow that breaks left-right symmetry of the body. However, the flow-sensing mechanism has long remained elusive. In this work, we show that immotile cilia at the node undergo asymmetric deformation along the dorsoventral axis in response to the flow. Application of mechanical stimuli to immotile cilia by optical tweezers induced calcium ion transients and degradation of Dand5 messenger RNA (mRNA) in the targeted cells. The Pkd2 channel protein was preferentially localized to the dorsal side of immotile cilia, and calcium ion transients were preferentially induced by mechanical stimuli directed toward the ventral side. Our results uncover the biophysical mechanism by which immotile cilia at the node sense the direction of fluid flow.
(MeSH Terms)

Contact
Takanobu A. Katoh, Toshihiro Omori, Hiroshi Hamada , The University of Tokyo, Tohoku University, Tata Instituite for Foundamental Research , Graduate School of Medicine, Graduate School of Biomedical Engineering, National Center for Biological Science , Cell Biology
Contributors


Dataset List of 295-Katoh-CiliumDyn

#
Dataset ID
Kind
Size
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SSBD:OMERO
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# 10794
Dataset Kind Image data
Dataset Size 407.2 MB
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SSBD:OMERO
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# 10795
Datast ID 2_MovieS3_after
Dataset Kind Image data
Dataset Size 1.2 GB
4D view
SSBD:OMERO
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# 10796
Datast ID 2_MovieS3_before
Dataset Kind Image data
Dataset Size 1.2 GB
4D view
SSBD:OMERO
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# 10797
Datast ID 3_FigS3A_UV_10s
Dataset Kind Image data
Dataset Size 198.0 MB
4D view
SSBD:OMERO
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# 10798
Datast ID 3_FigS3A_UV_20s
Dataset Kind Image data
Dataset Size 198.0 MB
4D view
SSBD:OMERO
Download BDML
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# 10799
Datast ID 3_FigS3A_UV_30s
Dataset Kind Image data
Dataset Size 198.0 MB
4D view
SSBD:OMERO
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# 10800
Datast ID 3_FigS3A_UV_40s
Dataset Kind Image data
Dataset Size 198.0 MB
4D view
SSBD:OMERO
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# 10801
Datast ID 3_FigS3A_UV_50s
Dataset Kind Image data
Dataset Size 198.0 MB
4D view
SSBD:OMERO
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# 10802
Datast ID 3_FigS3A_UV_before
Dataset Kind Image data
Dataset Size 601.1 MB
4D view
SSBD:OMERO
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# 10803
Dataset Kind Image data
Dataset Size 404.0 MB
4D view
SSBD:OMERO
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# 10804
Dataset Kind Image data
Dataset Size 404.0 MB
4D view
SSBD:OMERO
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# 10805
Dataset Kind Image data
Dataset Size 1.5 GB
4D view
SSBD:OMERO
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# 10806
Dataset Kind Image data
Dataset Size 202.4 MB
4D view
SSBD:OMERO
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# 10807
Dataset Kind Image data
Dataset Size 4.4 GB
4D view
SSBD:OMERO
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# 10808
Dataset Kind Image data
Dataset Size 301.4 MB
4D view
SSBD:OMERO
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# 10809
Datast ID 8_MovieS10_STED
Dataset Kind Image data
Dataset Size 578.1 MB
4D view
SSBD:OMERO
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# 10810
Datast ID 9_MovieS12_bending
Dataset Kind Image data
Dataset Size 254.5 MB
4D view
SSBD:OMERO
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# 10811
Dataset Kind Image data
Dataset Size 6.8 GB
4D view
SSBD:OMERO
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