calcium-mediated signaling, determination of left/right symmetry, detection of nodal flow
Cellular Component (CC)
non-motile cilium
Biological Imaging Method
time lapse microscopy, immunostaining, highly inclined and laminated optical sheet (HILO) microscopy, Spinning disk confocal microscopy, Spinning disk confocal microscope with custom-built optical pathway, AiryScan, Optical-tweezers, Spinning disk confocal microscopy with deconvolution, Whole-Cell FRAP (FRAPとは異なります。本論文で初めて報告した新規顕微鏡法です), STED, Calcium imaging
0.2180928 micrometer/slice, 0.1001360 micrometer/slice, 0.20 micrometer/pixel, 1000 micrometer/slice, NA
T scale
29.12 msec per time interval, 2 min per time interval, 100 msec per time interval, 1.122 sec per time interval, 6.550 sec per time interval, 0.292 sec per time interval, NA
Image Acquisition
Experiment type
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Microscope type
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Acquisition mode
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Contrast method
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Microscope model
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Detector model
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Objective model
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Filter set
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Related paper(s)
Takanobu A Katoh, Toshihiro Omori, Katsutoshi Mizuno, Xiaorei Sai, Katsura Minegishi, Yayoi Ikawa, Hiromi Nishimura, Takeshi Itabashi, Eriko Kajikawa, Sylvain Hiver, Atsuko H Iwane, Takuji Ishikawa, Yasushi Okada, Takayuki Nishizaka, Hiroshi Hamada (2023) Immotile cilia mechanically sense the direction of fluid flow for left-right determination., Science (New York, N.Y.), Volume 379, Number 6627, pp. 66-71
Published in 2023 Jan 6
(Electronic publication in Jan. 5, 2023, midnight )
(Abstract) Immotile cilia at the ventral node of mouse embryos are required for sensing leftward fluid flow that breaks left-right symmetry of the body. However, the flow-sensing mechanism has long remained elusive. In this work, we show that immotile cilia at the node undergo asymmetric deformation along the dorsoventral axis in response to the flow. Application of mechanical stimuli to immotile cilia by optical tweezers induced calcium ion transients and degradation of Dand5 messenger RNA (mRNA) in the targeted cells. The Pkd2 channel protein was preferentially localized to the dorsal side of immotile cilia, and calcium ion transients were preferentially induced by mechanical stimuli directed toward the ventral side. Our results uncover the biophysical mechanism by which immotile cilia at the node sense the direction of fluid flow.
Takanobu A. Katoh, Toshihiro Omori, Hiroshi Hamada
, The University of Tokyo, Tohoku University, Tata Instituite for Foundamental Research
, Graduate School of Medicine, Graduate School of Biomedical Engineering, National Center for Biological Science
, Cell Biology