Summary of 283-Kanadome-CellAdhesion

SSBD:database
SSBD:database URL
Title
Time-lapse imaging of reversible intercellular NCad interactions by INCIDER in HEK293T cells.
Description
-
Relase date
2023-07-20
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
2 ( Image datasets: 2, Quantitative data datasets: 0 )
Size of Datasets
28.6 MB ( Image datasets: 28.6 MB, Quantitative data datasets: 0 bytes )

Organism(s)
Homo sapiens
Cell lines(s)
HEK293T cell
Gene symbol(s)
NA
Protein name(s)
NA

Datatype
-
Molecular Function (MF)
cell-cell adhesion mediator activity, cadherin binding involved in cell-cell adhesion
Biological Process (BP)
cell adhesion
Cellular Component (CC)
Biological Imaging Method
time lapse microscopy
T scale
30 minutes of time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Takashi Kanadome, Kanehiro Hayashi, Yusuke Seto, Mototsugu Eiraku, Kazunori Nakajima, Takeharu Nagai, Tomoki Matsuda (2022) Development of intensiometric indicators for visualizing N-cadherin interaction across cells., Communications biology, Volume 5, Number 1, pp. 1065

Published in 2022 Oct 7 (Electronic publication in Oct. 7, 2022, midnight )

(Abstract) N-cadherin (NCad) is a classical cadherin that mediates cell-cell interactions in a Ca(2+)-dependent manner. NCad participates in various biological processes, from ontogenesis to higher brain functions, though the visualization of NCad interactions in living cells remains limited. Here, we present intensiometric NCad interaction indicators, named INCIDERs, that utilize dimerization-dependent fluorescent proteins. INCIDERs successfully visualize reversible NCad interactions across cells. Compared to FRET-based indicators, INCIDERs have a ~70-fold higher signal contrast, enabling clear identification of NCad interactions. In primary neuronal cells, NCad interactions are visualized between closely apposed processes. Furthermore, visualization of NCad interaction at cell adhesion sites in dense cell populations is achieved by two-photon microscopy. INCIDERs are useful tools in the spatiotemporal investigation of NCad interactions across cells; future research should evaluate the potential of INCIDERs in mapping complex three-dimensional architectures in multi-cellular systems.
(MeSH Terms)

Contact
Tomoki Matsuda , Osaka University , SANKEN (The Institute of Scientific and Industrial Research) , Department of Biomolecular Science and Engineering
Contributors


Dataset List of 283-Kanadome-CellAdhesion

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
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# 10779
Dataset Kind Image data
Dataset Size 15.9 MB
4D view
SSBD:OMERO
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# 10780
Dataset Kind Image data
Dataset Size 12.7 MB
4D view
SSBD:OMERO
Download BDML
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