Summary of 246-Hung-CircadianRhythm

SSBD:database
SSBD:database URL
Title
Time-series images of daily patterns of wake, NREM, and REM sleep in control or CRF-Cre/Bmal1flox/flox mice under LD and DD.
Description
-
Relase date
2023-05-11
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
4 ( Image datasets: 4, Quantitative data datasets: 0 )
Size of Datasets
157.2 GB ( Image datasets: 157.2 GB, Quantitative data datasets: 0 bytes )

Organism(s)
Mus musculus
Gene symbol(s)
Bmal1
Protein name(s)
NA

Datatype
-
Molecular Function (MF)
Biological Process (BP)
circadian rhythm, wakefulness, circadian sleep/wake cycle
Cellular Component (CC)
Biological Imaging Method
Animal behavior was monitored using a charge-coupled device video camera (SPK-E700CHP1, Keiyo Techno) and recorded using an infrared activity sensor (Kissei Comtec).
X scale
camera1: 0.04 centimeter/pixel, camera2: 0.08 centimeter/pixel, camera3: 0.05 centimeter/pixel, camera4: 0.05 centimeter/pixel
Y scale
camera1: 0.04 centimeter/pixel, camera2: 0.08 centimeter/pixel, camera3: 0.05 centimeter/pixel, camera4: 0.05 centimeter/pixel
Z scale
-
T scale
30 frame per second

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Chi Jung Hung, Akihiro Yamanaka, Daisuke Ono (2021) Conditional Knockout of Bmal1 in Corticotropin-Releasing Factor Neurons Does Not Alter Sleep-Wake Rhythm in Mice., Frontiers in neuroscience, Volume 15, pp. 808754

Published in 2021 (Electronic publication in Feb. 18, 2022, midnight )

(Abstract) Sleep and wakefulness are regulated by both the homeostatic mechanism and circadian clock. In mammals, the central circadian clock, the suprachiasmatic nucleus, in the hypothalamus plays a crucial role in the timing of physiology and behavior. Recently, we found that the circadian regulation of wakefulness was transmitted via corticotropin-releasing factor (CRF) neurons in the paraventricular nucleus of the hypothalamus to orexin neurons in the lateral hypothalamus. However, it is still unclear how the molecular clock in the CRF neurons contributes to the regulation of sleep and wakefulness. In the present study, we established CRF neuron-specific Bmal1-deficient mice and measured locomotor activity or electroencephalography and electromyography. We found that these mice showed normal circadian locomotor activity rhythms in both light-dark cycle and constant darkness. Furthermore, they showed normal daily patterns of sleep and wakefulness. These results suggest that Bmal1 in CRF neurons has no effect on either circadian locomotor activity or sleep and wakefulness.

Contact
Daisuke Ono , Nagoya University , Research Institute of Environmental Medicine , Department of Neuroscience II
Contributors


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Dataset List of 246-Hung-CircadianRhythm

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
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# 10670
Datast ID Fig4_20200519-0002
Dataset Kind Image data
Dataset Size 40.4 GB
4D view
SSBD:OMERO
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# 10671
Dataset Kind Image data
Dataset Size 39.8 GB
4D view
SSBD:OMERO
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# 10672
Dataset Kind Image data
Dataset Size 38.5 GB
4D view
SSBD:OMERO
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# 10673
Dataset Kind Image data
Dataset Size 38.5 GB
4D view
SSBD:OMERO
Download BDML
Download Image data