Summary of 220-Imanishi-ROCKActivity

SSBD:database
SSBD:database URL
Title
Time lapse images of the spatiotemporal regulation of ROCK activity in mice live blood vessels.
Description
-
Relase date
2023-02-16
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
134 ( Image datasets: 134, Quantitative data datasets: 0 )
Size of Datasets
17.1 GB ( Image datasets: 17.1 GB, Quantitative data datasets: 0 bytes )

Organism(s)
Mus musculus
Gene symbol(s)
NA
Protein name(s)
ERK, PKA, ROCK
Reporter(s)
RGECO1

Datatype
-
Molecular Function (MF)
Rho-dependent protein serine/threonine kinase activity
Biological Process (BP)
Cellular Component (CC)
Biological Imaging Method
time lapse microscopy, two-photon excitation microscopy, FRET
X scale
0.284 micrometer/pixel, 0.349 micrometer/pixel, 0.262 micrometer/pixel, 0.324 micrometer/pixel, 0.29 micrometer/pixel, 0.331 micrometer/pixel, 0.378 micrometer/pixel, 0.2842157 micrometer/pixel, 0.497 micrometer/pixel
Y scale
0.284 micrometer/pixel, 0.349 micrometer/pixel, 0.262 micrometer/pixel, 0.324 micrometer/pixel, 0.29 micrometer/pixel, 0.331 micrometer/pixel, 0.378 micrometer/pixel, 0.2842157 micrometer/pixel, 0.497 micrometer/pixel
Z scale
1 micrometer/slice, 0.3461841 micrometer/slice
T scale
5.36 seconds per frame, 5.51 seconds per frame, 0.64 second per frame, 0.85 second per frame, 6.15 seconds per frame, 5.63 seconds per frame, 7.22 seconds per frame, 0.63 second per frame, 0.6 second per frame, 5.54 seconds per frame, 5.6 seconds per frame, 6.73 seconds per frame, 5.5 seconds per frame, 2.83 second per frame, 5.46 seconds per frame, 3.34 second per frame, 0.57 second per frame, 6.64 seconds per frame, 0.56 second per frame, 0.58 second per frame, 5.57 seconds per frame, 0.65 second per frame, 5.03 seconds per frame, 0.7 second per frame, 0.71 second per frame, NA, 5.08 seconds per frame, 5.49 seconds per frame, 5.33 seconds per frame

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Ayako Imanishi, Hiroshi Ichise, Chuyun Fan, Yasuaki Nakagawa, Koichiro Kuwahara, Kenta Sumiyama, Michiyuki Matsuda, Kenta Terai (2021) Visualization of Spatially-Controlled Vasospasm by Sympathetic Nerve-Mediated ROCK Activation., The American journal of pathology, Volume 191, Number 1, pp. 194-203

Published in 2021 Jan (Electronic publication in Oct. 16, 2020, midnight )

(Abstract) Contraction of vascular smooth muscle is regulated primarily by calcium concentration and secondarily by ROCK activity within the cells. In contrast to the wealth of information regarding regulation of calcium concentration, little is known about the spatiotemporal regulation of ROCK activity in live blood vessels. Here, we report ROCK activation in subcutaneous arterioles in a transgenic mouse line that expresses a genetically encoded ROCK biosensor based on the principle of Frster resonance energy transfer by two-photon excitation in vivo imaging. Rapid vasospasm was induced upon laser ablation of arterioles, concomitant with a transient increase in calcium concentration in arteriolar smooth muscles. Unlike the increase in calcium concentration, vasoconstriction and ROCK activation continued for several minutes after irradiation. Both the ROCK inhibitor, fasudil, and the ganglionic nicotinic acetylcholine receptor blocker, hexamethonium, inhibited laser-induced ROCK activation and reduced the duration of vasospasm at the segments distant from the irradiated point. These observations suggest that vasoconstriction is initially triggered by a rapid surge of cytoplasmic calcium and then maintained by sympathetic nerve-mediated ROCK activation.
(MeSH Terms)

Contact
Kenta Terai , kyoto University , Graduate School of Medicine , Department of Pathology and Biology of Diseases
Contributors


Dataset List of 220-Imanishi-ROCKActivity

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Dataset ID
Kind
Size
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# 10185
Dataset Kind Image data
Dataset Size 652.8 MB
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# 10186
Datast ID Fig1D_1a_70parcent
Dataset Kind Image data
Dataset Size 129.4 MB
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# 10187
Datast ID Fig1D_5b_70parcent
Dataset Kind Image data
Dataset Size 129.4 MB
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# 10188
Datast ID Fig1D_6b_70parcent
Dataset Kind Image data
Dataset Size 129.4 MB
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# 10189
Datast ID Fig1D_7b_70parcent
Dataset Kind Image data
Dataset Size 129.4 MB
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# 10190
Datast ID Fig1D_8b_70parcent
Dataset Kind Image data
Dataset Size 129.4 MB
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# 10191
Datast ID Fig2B_6b_70parcent
Dataset Kind Image data
Dataset Size 10.8 MB
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SSBD:OMERO
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# 10192
Datast ID Fig2C_180724_ERK
Dataset Kind Image data
Dataset Size 346.7 MB
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SSBD:OMERO
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# 10193
Datast ID Fig2C_181122_PKA
Dataset Kind Image data
Dataset Size 194.0 MB
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# 10194
Dataset Kind Image data
Dataset Size 10.8 MB
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# 10195
Dataset Kind Image data
Dataset Size 133.0 MB
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# 10196
Dataset Kind Image data
Dataset Size 32.8 MB
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# 10197
Dataset Kind Image data
Dataset Size 32.7 MB
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# 10198
Dataset Kind Image data
Dataset Size 10.8 MB
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Dataset Kind Image data
Dataset Size 10.8 MB
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Dataset Kind Image data
Dataset Size 10.8 MB
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Dataset Kind Image data
Dataset Size 10.8 MB
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Dataset Kind Image data
Dataset Size 10.8 MB
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Dataset Kind Image data
Dataset Size 10.8 MB
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Dataset Kind Image data
Dataset Size 10.8 MB
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# 10205
Dataset Kind Image data
Dataset Size 10.8 MB
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# 10206
Dataset Kind Image data
Dataset Size 10.8 MB
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# 10207
Dataset Kind Image data
Dataset Size 20.4 MB
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Dataset Kind Image data
Dataset Size 20.4 MB
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Dataset Kind Image data
Dataset Size 20.4 MB
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Dataset Kind Image data
Dataset Size 20.4 MB
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# 10211
Dataset Kind Image data
Dataset Size 20.9 MB
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# 10212
Dataset Kind Image data
Dataset Size 431.5 MB
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# 10213
Dataset Kind Image data
Dataset Size 10.8 MB
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# 10214
Dataset Kind Image data
Dataset Size 10.8 MB
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# 10215
Dataset Kind Image data
Dataset Size 10.5 MB
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# 10216
Dataset Kind Image data
Dataset Size 10.8 MB
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# 10217
Dataset Kind Image data
Dataset Size 10.8 MB
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# 10218
Dataset Kind Image data
Dataset Size 10.5 MB
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# 10219
Dataset Kind Image data
Dataset Size 10.5 MB
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# 10220
Dataset Kind Image data
Dataset Size 23.7 MB
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# 10221
Dataset Kind Image data
Dataset Size 20.4 MB
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# 10222
Dataset Kind Image data
Dataset Size 20.9 MB
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# 10223
Dataset Kind Image data
Dataset Size 20.9 MB
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# 10224
Dataset Kind Image data
Dataset Size 20.9 MB
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# 10225
Dataset Kind Image data
Dataset Size 20.4 MB
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# 10226
Dataset Kind Image data
Dataset Size 20.4 MB
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# 10227
Dataset Kind Image data
Dataset Size 20.4 MB
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# 10228
Dataset Kind Image data
Dataset Size 20.4 MB
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# 10229
Dataset Kind Image data
Dataset Size 346.7 MB
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# 10230
Datast ID Fig3A_180912_Fas
Dataset Kind Image data
Dataset Size 431.5 MB
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# 10231
Dataset Kind Image data
Dataset Size 230.7 MB
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# 10232
Dataset Kind Image data
Dataset Size 231.2 MB
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# 10233
Dataset Kind Image data
Dataset Size 346.7 MB
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# 10234
Dataset Kind Image data
Dataset Size 346.7 MB
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# 10235
Dataset Kind Image data
Dataset Size 431.5 MB
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# 10236
Dataset Kind Image data
Dataset Size 693.4 MB
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# 10237
Dataset Kind Image data
Dataset Size 346.7 MB
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# 10238
Dataset Kind Image data
Dataset Size 1.0 GB
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# 10239
Dataset Kind Image data
Dataset Size 231.2 MB
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# 10240
Dataset Kind Image data
Dataset Size 230.7 MB
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# 10241
Dataset Kind Image data
Dataset Size 231.2 MB
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# 10242
Dataset Kind Image data
Dataset Size 231.2 MB
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# 10243
Dataset Kind Image data
Dataset Size 346.7 MB
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# 10244
Dataset Kind Image data
Dataset Size 346.7 MB
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# 10245
Dataset Kind Image data
Dataset Size 179.8 MB
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# 10246
Dataset Kind Image data
Dataset Size 230.7 MB
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# 10247
Dataset Kind Image data
Dataset Size 231.2 MB
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# 10248
Dataset Kind Image data
Dataset Size 231.2 MB
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# 10249
Dataset Kind Image data
Dataset Size 133.0 MB
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# 10250
Dataset Kind Image data
Dataset Size 79.1 MB
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# 10251
Dataset Kind Image data
Dataset Size 129.4 MB
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# 10252
Dataset Kind Image data
Dataset Size 129.4 MB
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# 10253
Dataset Kind Image data
Dataset Size 231.2 MB
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# 10254
Dataset Kind Image data
Dataset Size 231.2 MB
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# 10255
Dataset Kind Image data
Dataset Size 230.7 MB
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# 10256
Dataset Kind Image data
Dataset Size 230.7 MB
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# 10257
Dataset Kind Image data
Dataset Size 231.2 MB
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# 10258
Dataset Kind Image data
Dataset Size 231.2 MB
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# 10259
Dataset Kind Image data
Dataset Size 346.7 MB
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# 10260
Dataset Kind Image data
Dataset Size 346.7 MB
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# 10261
Dataset Kind Image data
Dataset Size 431.5 MB
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# 10262
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Dataset Size 431.5 MB
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# 10263
Dataset Kind Image data
Dataset Size 64.5 MB
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# 10264
Dataset Kind Image data
Dataset Size 431.5 MB
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# 10265
Dataset Kind Image data
Dataset Size 430.5 MB
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# 10266
Datast ID Fig4A_200115_10c
Dataset Kind Image data
Dataset Size 10.5 MB
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# 10267
Datast ID Fig4B_200205_10e
Dataset Kind Image data
Dataset Size 10.8 MB
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# 10268
Dataset Kind Image data
Dataset Size 160.9 MB
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# 10269
Dataset Kind Image data
Dataset Size 129.2 MB
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# 10270
Dataset Kind Image data
Dataset Size 129.2 MB
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# 10271
Dataset Kind Image data
Dataset Size 129.2 MB
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# 10272
Dataset Kind Image data
Dataset Size 129.2 MB
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# 10273
Dataset Kind Image data
Dataset Size 26.6 MB
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# 10274
Dataset Kind Image data
Dataset Size 180.3 MB
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# 10275
Dataset Kind Image data
Dataset Size 129.2 MB
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# 10276
Dataset Kind Image data
Dataset Size 129.2 MB
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# 10277
Dataset Kind Image data
Dataset Size 129.2 MB
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# 10278
Dataset Kind Image data
Dataset Size 128.6 MB
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# 10279
Dataset Kind Image data
Dataset Size 129.2 MB
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# 10280
Dataset Kind Image data
Dataset Size 21.5 MB
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# 10281
Dataset Kind Image data
Dataset Size 20.4 MB
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# 10282
Dataset Kind Image data
Dataset Size 20.4 MB
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# 10283
Dataset Kind Image data
Dataset Size 20.4 MB
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# 10284
Dataset Kind Image data
Dataset Size 20.4 MB
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# 10285
Dataset Kind Image data
Dataset Size 20.4 MB
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# 10286
Dataset Kind Image data
Dataset Size 20.4 MB
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# 10287
Dataset Kind Image data
Dataset Size 263.6 MB
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# 10288
Dataset Kind Image data
Dataset Size 263.6 MB
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# 10289
Dataset Kind Image data
Dataset Size 265.0 MB
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# 10290
Dataset Kind Image data
Dataset Size 263.6 MB
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# 10291
Dataset Kind Image data
Dataset Size 10.8 MB
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# 10292
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Dataset Size 10.8 MB
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# 10293
Dataset Kind Image data
Dataset Size 10.8 MB
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# 10294
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Dataset Size 10.8 MB
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# 10295
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Dataset Size 10.8 MB
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# 10296
Dataset Kind Image data
Dataset Size 10.8 MB
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# 10297
Dataset Kind Image data
Dataset Size 10.8 MB
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# 10298
Dataset Kind Image data
Dataset Size 10.8 MB
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# 10299
Dataset Kind Image data
Dataset Size 10.5 MB
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# 10300
Dataset Kind Image data
Dataset Size 10.5 MB
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# 10301
Dataset Kind Image data
Dataset Size 10.8 MB
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# 10302
Dataset Kind Image data
Dataset Size 10.8 MB
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# 10303
Dataset Kind Image data
Dataset Size 8.5 MB
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# 10304
Dataset Kind Image data
Dataset Size 21.5 MB
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# 10305
Dataset Kind Image data
Dataset Size 20.4 MB
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# 10306
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Dataset Size 20.4 MB
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# 10307
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Dataset Size 20.4 MB
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# 10308
Dataset Kind Image data
Dataset Size 20.4 MB
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# 10309
Dataset Kind Image data
Dataset Size 20.4 MB
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# 10310
Dataset Kind Image data
Dataset Size 20.4 MB
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# 10311
Dataset Kind Image data
Dataset Size 26.6 MB
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# 10312
Dataset Kind Image data
Dataset Size 80.8 MB
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# 10313
Dataset Kind Image data
Dataset Size 8.1 MB
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# 10314
Datast ID SuppFigS1C_kidney
Dataset Kind Image data
Dataset Size 10.1 MB
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# 10315
Datast ID SuppFigS1C_liver
Dataset Kind Image data
Dataset Size 10.1 MB
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# 10316
Dataset Kind Image data
Dataset Size 44.4 MB
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# 10317
Dataset Kind Image data
Dataset Size 10.1 MB
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# 10318
Datast ID SuppFigS1C_spleen
Dataset Kind Image data
Dataset Size 10.1 MB
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