Summary of 190-Iguchi-SpineDyn

SSBD:database
SSBD:database URL
Title
Living images of dendrites expressing DsRed2 and PSD-95-EGFP in control mice and socially isolated mice.
Description
-
Relase date
2022-03-31
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
4 ( Image datasets: 4, Quantitative data datasets: 0 )
Size of Datasets
309.2 MB ( Image datasets: 309.2 MB, Quantitative data datasets: 0 bytes )

Organism(s)
Mus musculus
Gene symbol(s)
NA
Protein name(s)
PSD-95
Protein tag(s)
EGFP
Reporter(s)
DsRed2

Datatype
-
Molecular Function (MF)
-
Biological Process (BP)
response to social isolation
Cellular Component (CC)
dendritic spine
Biological Imaging Method
two-photon laser scanning microscopy
X scale
0.15micrometer/pixel
Y scale
0.15micrometer/pixel
Z scale
0.75 micrometer/slice
T scale
-

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Risa Iguchi, Shinji Tanaka, Shigeo Okabe (2020) Neonatal social isolation increases the proportion of the immature spines in the layer 2/3 pyramidal neurons of the somatosensory cortex., Neuroscience research, Volume 154, pp. 27-34

Published in 2020 May (Electronic publication in June 18, 2019, midnight )

(Abstract) Social isolation during the juvenile period is postulated to leave specific sequelae, such as attention deficits and emotion recognition. Miswiring of the cortical neuronal circuit during postnatal development may underlie such behavioral impairments, but the details of the circuit-level impairment associated with social isolation have not yet been clarified. In this study, we evaluated the possibility that environmental factors may induce alternation in spine characteristics and dynamics. We isolated mice from the mother and siblings from postnatal day 7 to 11 for 6h per day. Both dynamics and structural properties of spines in the layer 2/3 pyramidal neurons of the somatosensory cortex were measured at postnatal 3 weeks by in vivo two-photon microscopy. We found decrease in the ratio of PSD-95-positive dendritic spines in the mice after social isolation. These mice did not show alteration in spine dynamics. Those results suggest that the neonatal social isolation results in less mature spines, with normal rate of their turnover, which is distinct from spine phenotype seen in multiple models of autism spectrum disorders.
(MeSH Terms)

Contact
Shigeo Okabe , the University of Tokyo, Tokyo , Department of Cellular Neurobiology, Graduate School of Medicine and Faculty of Medicine
Contributors


Dataset List of 190-Iguchi-SpineDyn

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
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# 7419
Dataset Kind Image data
Dataset Size 69.0 MB
4D view
SSBD:OMERO
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# 7420
Dataset Kind Image data
Dataset Size 81.1 MB
4D view
SSBD:OMERO
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# 7421
Dataset Kind Image data
Dataset Size 80.1 MB
4D view
SSBD:OMERO
Download BDML
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# 7422
Dataset Kind Image data
Dataset Size 79.1 MB
4D view
SSBD:OMERO
Download BDML
Download Image data