Summary of 136-Matsumiya-ESCellDyn

SSBD:database
SSBD:database URL
Title
Time-lapse imaging of Hes7 promoter-driven luciferase activity in mouse embryos or presomitic mesoderm-like tissues (iPSM) induced from mouse ES cells
Description
-
Relase date
2019-11-20
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
9 ( Image datasets: 9, Quantitative data datasets: 0 )
Size of Datasets
216.6 MB ( Image datasets: 216.6 MB, Quantitative data datasets: 0 bytes )

Organism(s)
M. musculus
Strain(s)
mouse embryonic stem cells (E14TG2a)
Gene symbol(s)
Hes7
Protein name(s)
NA

Datatype
Hes7 expression dynamics
Molecular Function (MF)
Biological Process (BP)
segmentation, somite development
Cellular Component (CC)
-
Biological Imaging Method
-
XYZ Scale
XY: 12.4 micrometer/pixel, Z: NA, XY: 24.8 micrometer/pixel, Z: NA, XY: 1.24 micrometer/pixel, Z: NA, XY: 2.48 micrometer/pixel, Z: NA
T scale
270 second for each time interval, 100 millisecond for each time interval

Image Acquisition
Experiment type
TimeLapse
Microscope type
Other
Acquisition mode
Other
Contrast method
Fluorescence
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Marina Matsumiya, Takehito Tomita, Kumiko Yoshioka-Kobayashi, Akihiro Isomura, Ryoichiro Kageyama (2018) ES cell-derived presomitic mesoderm-like tissues for analysis of synchronized oscillations in the segmentation clock., Development (Cambridge, England), Volume 145, Number 4

Published in 2018 Feb 14 (Electronic publication in Feb. 14, 2018, midnight )

(Abstract) Somites are periodically formed by segmentation of the anterior parts of the presomitic mesoderm (PSM). In the mouse embryo, this periodicity is controlled by the segmentation clock gene Hes7, which exhibits wave-like oscillatory expression in the PSM. Despite intensive studies, the exact mechanism of such synchronous oscillatory dynamics of Hes7 expression still remains to be analyzed. Detailed analysis of the segmentation clock has been hampered because it requires the use of live embryos, and establishment of an in vitro culture system would facilitate such analyses. Here, we established a simple and efficient method to generate mouse ES cell-derived PSM-like tissues, in which Hes7 expression oscillates like traveling waves. In these tissues, Hes7 oscillation is synchronized between neighboring cells, and the posterior-anterior axis is self-organized as the central-peripheral axis. This method is applicable to chemical-library screening and will facilitate the analysis of the molecular nature of the segmentation clock.
(MeSH Terms)

Contact
Ryoichiro Kageyama , Kyoto University , Institute for Frontier Life and Medical Sciences
Contributors
Marina Matsumiya, Takehito Tomita, Kumiko Yoshioka-Kobayashi, Akihiro Isomura, Ryoichiro Kageyama


Dataset List of 136-Matsumiya-ESCellDyn

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
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# 4983
Datast ID Fig3_iPSM_BF
Dataset Kind Image data
Dataset Size 32.4 MB
4D view
SSBD:OMERO
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# 4984
Datast ID Fig3_iPSM_chemi
Dataset Kind Image data
Dataset Size 64.5 MB
4D view
SSBD:OMERO
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# 4985
Datast ID Fig3_iPSM_mCh
Dataset Kind Image data
Dataset Size 8.0 MB
4D view
SSBD:OMERO
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# 4986
Datast ID Fig7_DMSO-BF
Dataset Kind Image data
Dataset Size 6.8 MB
4D view
SSBD:OMERO
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# 4987
Datast ID Fig7_DMSO-Chemi
Dataset Kind Image data
Dataset Size 6.8 MB
4D view
SSBD:OMERO
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# 4988
Datast ID Fig7_IBET-BF
Dataset Kind Image data
Dataset Size 6.8 MB
4D view
SSBD:OMERO
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# 4989
Datast ID Fig7_IBET-Chemi
Dataset Kind Image data
Dataset Size 6.8 MB
4D view
SSBD:OMERO
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# 4990
Datast ID Fig7_J_BF
Dataset Kind Image data
Dataset Size 36.2 MB
4D view
SSBD:OMERO
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# 4991
Datast ID Fig7_J_Chemi
Dataset Kind Image data
Dataset Size 48.2 MB
4D view
SSBD:OMERO
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