SSBD:database v3

411-Yoshinari-BorateTransporter

Project

Title

3D-time-lapse images of vacuolar sorting of BOR1

Description

Plants maintain nutrient homeostasis by controlling the activities and abundance of nutrient transporters. In Arabidopsis thaliana, the borate (B) transporter BOR1 plays a role in the efficient translocation of B under low-B conditions. BOR1 undergoes polyubiquitination in the presence of sufficient B and is then transported to the vacuole via multivesicular bodies (MVBs) to prevent B accumulation in tissues at a toxic level. A previous study indicated that BOR1 physically interacts with mu subunits of adaptor protein complexes AP-3 and AP-4, both involved in vacuolar sorting pathways. In this study, we investigated the roles of AP-3 and AP-4 subunits in BOR1 trafficking in Arabidopsis. The lack of AP-3 subunits did not affect either vacuolar sorting or polar localization of BOR1-GFP, whereas the absence of AP-4 subunits resulted in a delay in high-B-induced vacuolar sorting without affecting polar localization. Super-resolution microscopy revealed a rapid sorting of BOR1-GFP into AP-4-positive spots in the trans-Golgi network (TGN) upon high-B supply. These results indicate that AP-4 is involved in sequestration of ubiquitinated BOR1 into a TGN-specific subdomain 'vacuolar-trafficking zone', and is required for efficient sorting of MVB and vacuole. Their findings have thus helped elucidate the rapid vacuolar sorting process facilitated by AP-4 in plant nutrient transporters.

License

CC BY 4.0

Released at

Oct. 20, 2025, midnight

Updated at

Funding

JSPS Grant-in-Aid for Scientific Research on Innovative Areas (19H05763 to J.T.), JSPS Grant-in-Aid for Scientific Research (A) (19H00934 to J.T.), JSPS Grant-in-Aid for Young Scientists (A) (26712007 to J.T.), Grant-in-Aid for Scientific Research(B) (22H02643 to T.U.), Grant-in-Aid for Challenging Research (Exploratory) (22K19327 to T.U.), JST CREST (JPMJCR20E5 to T.U.), JST PRESTO (JPMJPR22D9 to A.Y.) and Asahi Glass Foun- dation (to T.U.).

# of image datasets

11

Papers

Papers

Paper information: Yoshinari A, Shimizu Y, Hosokawa T, Nakano A, Uemura T, Takano J. Rapid Vacuolar Sorting of the Borate Transporter BOR1 Requires the Adaptor Protein Complex AP-4 in Arabidopsis. Plant Cell Physiol. 2024 Dec 6;65(11):1801-1811.,
Pubmed ID: 39215599,
DOI: https://doi.org/10.1093/pcp/pcae096,
URL: https://pubmed.ncbi.nlm.nih.gov/39215599/

Contacts

Contacts

Junpei Takano (Contact) , Graduate School of Life and Environmental Sciences , Osaka Prefecture University
Akira Yoshinari (Image data contributor) , Institute of Transformative Bio-Molecules (WPI-ITbM) , Nagoya University
Yutaro Shimizu (Image data contributor) , Center for Advanced Photonics , RIKEN
Takuya Hosokawa (Image data contributor) , Graduate School of Life and Environmental Sciences , Osaka Prefecture University

Biosamples

Organisms

Arabidopsis thaliana (NCBI:txid3702)

Strains

Col-0

Cells

epidermal cell (CL_0000362)

Cell lines

Ontologies

UBERON