411-Yoshinari-BorateTransporter
Author reviewed3D-time-lapse images of vacuolar sorting of BOR1
Project ID
411-Yoshinari-BorateTransporter
Project SID
411
Title
3D-time-lapse images of vacuolar sorting of BOR1
Description
Plants maintain nutrient homeostasis by controlling the activities and abundance of nutrient transporters. In Arabidopsis thaliana, the borate (B) transporter BOR1 plays a role in the efficient translocation of B under low-B conditions. BOR1 undergoes polyubiquitination in the presence of sufficient B and is then transported to the vacuole via multivesicular bodies (MVBs) to prevent B accumulation in tissues at a toxic level. A previous study indicated that BOR1 physically interacts with mu subunits of adaptor protein complexes AP-3 and AP-4, both involved in vacuolar sorting pathways. In this study, we investigated the roles of AP-3 and AP-4 subunits in BOR1 trafficking in Arabidopsis. The lack of AP-3 subunits did not affect either vacuolar sorting or polar localization of BOR1-GFP, whereas the absence of AP-4 subunits resulted in a delay in high-B-induced vacuolar sorting without affecting polar localization. Super-resolution microscopy revealed a rapid sorting of BOR1-GFP into AP-4-positive spots in the trans-Golgi network (TGN) upon high-B supply. These results indicate that AP-4 is involved in sequestration of ubiquitinated BOR1 into a TGN-specific subdomain 'vacuolar-trafficking zone', and is required for efficient sorting of MVB and vacuole. Their findings have thus helped elucidate the rapid vacuolar sorting process facilitated by AP-4 in plant nutrient transporters.
Submission Date
2025-04-10
Opened Date
-
Release Date
2025-11-28
Update Date
-
Kind
Image datasets
License
Project URL
-
Project DOI
-
Metadata Version
-
Template Version
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Funding Information
JSPS Grant-in-Aid for Scientific Research on Innovative Areas (19H05763 to J.T.), JSPS Grant-in-Aid for Scientific Research
(A) (19H00934 to J.T.), JSPS Grant-in-Aid for Young Scientists
(A) (26712007 to J.T.), Grant-in-Aid for Scientific Research(B)
(22H02643 to T.U.), Grant-in-Aid for Challenging Research
(Exploratory) (22K19327 to T.U.), JST CREST (JPMJCR20E5 to T.U.), JST PRESTO (JPMJPR22D9 to A.Y.) and Asahi Glass Foun-
dation (to T.U.).
Total
163 files / 1.3 GB
Image datasets
zipped 11 files / 253.2 MB, raw image 62 files / 397.1 MB, total 73 files / 650.2 MB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Biosample
Organism
Arabidopsis thaliana
(
NCBITaxon:3702
)
Strain
Cell
epidermal cell
(
CL:0000362
)
Cell line
-
MeSH
Ontology
Anatomical Entity
root
root epidermis
(
PO:0006036
)
UBERON
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Biological Process
vesicle-mediated transport
intracellular borate homeostasis
(
GO:0160070
)
Cellular Component
vesicle membrane
plasma membrane
vacuole
(
GO:0005773
)
Molecular Function
active borate transmembrane transporter activity
(
GO:0046715
)
Medical Subject Headings
Imaging Method
Method involved in biological imaging
confocal microscopy
(
FBbi:00000251
)
time lapse microscopy
(
FBbi:00000249
)
Paper DOI / Paper URL
People
Contact
Junpei Takano
Osaka Prefecture University
Graduate School of Life and Environmental Sciences
-
-
Imaging dataset contributor
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Quantitative dataset contributor
Akira Yoshinari (Nagoya University)
Yutaro Shimizu (RIKEN)
Takuya Hosokawa (Osaka Prefecture University)
Image datasets
11
Quantitative datasets
0
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
Time-series images of BOR1-GFP in the root epidermis of wild-type Arabidopsis after high borate application
Time-series images of BOR1-GFP in the root epidermis of ap4b-2 mutant Arabidopsis after high borate application
Time-series images of BOR1-GFP in the root epidermis of ap4m-3 mutant Arabidopsis after high borate application
Time-series images of BOR1-GFP in the root epidermis of ap4m-4 mutant Arabidopsis after high borate application
Time-series images of BOR1-GFP in the root epidermis of wild-type Arabidopsis expressing BOR1-GFP construct without five prime untranslated region
Time-series images of BOR1-GFP in the root epidermis of ap3b-1 mutant Arabidopsis expressing BOR1-GFP construct without five prime untranslated region
Time-series images of BOR1-GFP in the root epidermis of wild-type Arabidopsis expressing BOR1-GFP construct with five prime untranslated region
Time-series images of BOR1-GFP in the root epidermis of ap3m-1 mutant Arabidopsis expressing BOR1-GFP construct with five prime untranslated region
Z-series images of BOR1-GFP, AP4M-TagRFP and iRFP-SYP61 in Arabidopsis root obtained by SCLIM
Z-series images of BOR1-GFP, AP1M2-TagRFP and AP4M-iRFP in Arabidopsis root obtained by SCLIM
Z-series and time-lapse SCLIM images of BOR1-GFP and AP4M-TagRFP in the root epidermal cells after treatment with high concentration of boric acid