Dataset Fig4A_VB_Before-infect
Project
393-Fujimoto-CoV2Infect
Title
SARS-CoV-2 infection to the co-culture of bronchial organoids and vascular beds in a microfluidic device
Description
Blood vessels show various COVID-19-related conditions including thrombosis and cytokine propagation. Existing in vitro blood vessel models cannot represent the consequent changes in the vascular structure or determine the initial infection site, making it difficult to evaluate how epithelial and endothelial tissues are damaged. Here, the authors developed a microphysiological system (MPS) that co-culture the bronchial organoids and the vascular bed to analyze infection site and interactions. In this system, virus-infected organoids caused damage in vascular structure. However, vasculature was not damaged or infected when the virus was directly introduced to vascular bed. The knockout of interferon-related genes and inhibition of the JAK/STAT pathway reduced the vascular damage, indicating the protective effect of interferon response suppression. The results demonstrate selective infection of bronchial epithelial cells and vascular damage by cytokines and also indicate the applicability of MPS to investigate how the infection influences vascular structure and functions.
Funding
KAKENHI (Grant-in-Aid for Scientific Research) on Priority Areas ‘Systems Genomics’ [17017038]
Title
Fluorescent images of the vascular bed before infection with mock virus or SARS-CoV-2 virus
Description
Fluorescent images of the vascular bed 4-days before infection with mock virus or SARS-CoV-2 virus. Bronchial organoids and vascular bed composed of HUVECs (Human Umbilical Vein Endothelial Cells) were co-cultured in a special 3D microfluidic device. GFP-expressing HUVECs were used to observe the morphology of vascular bed. Twenty independent images are deposited.
Description
Co-culture of bronchial organoids, which were derived from normal human bronchial epithelial
cells, and vascular beds, which were composed from GFP-Expressing Human Umbilical Vein Endothelial Cells, in a special microfluidic device.
Intrinsic variables
The commercial HUVECSs expressing GFP were used.
GFP-HUVECs
(Angio-Proteomie, cAP-0001, Boston, MA, USA)
Gene ontology: Biological processes
Gene ontology: Cellular components
Gene ontology: Molecular functions
Dimensions
1920x1200x1x1x1
Visualization methods
Green fluorescent protein from Aequorea
Contrast enhancing methods
Resolution enhancing methods
Sample preparation methods
living tissue
Body
Olympus-Evident CKX53
Detector
Olympus-Evident DP74
Objective
Olympus-Evident CACHN10XIPC