SSBD:database

Project ID

379-Okubo-bilaminoid

Project SID

379

Title

3D images of bilaminar organoid generated from co-culture of human pluripotent stem cells (hPSC) and hPSC-derived hypoblast-like cells

Description

Recently, several studies using cultures of human embryos together with single-cell RNA-seq analyses have revealed differences between humans and mice, necessitating the study of human embryos. Despite the importance of human embryology, ethical and legal restrictions have limited post-implantation-stage studies. Thus, recent efforts have focused on developing in vitro self-organizing models using human stem cells. In this paper, the authors report genetic and non-genetic approaches to generate authentic hypoblast cells (naive hPSC-derived hypoblast-like cells (nHyCs))-known to give rise to one of the two extraembryonic tissues essential for embryonic development-from naive human pluripotent stem cells (hPSCs). Their nHyCs spontaneously assemble with naive hPSCs to form a three-dimensional bilaminar structure (bilaminoids) with a pro-amniotic-like cavity. In the presence of additional naive hPSC-derived analogues of the second extraembryonic tissue, the trophectoderm, the efficiency of bilaminoid formation increases from 20% to 40%, and the epiblast within the bilaminoids continues to develop in response to trophectoderm-secreted IL-6. Furthermore, they show that bilaminoids robustly recapitulate the patterning of the anterior-posterior axis and the formation of cells reflecting the pregastrula stage, the emergence of which can be shaped by genetically manipulating the DKK1/OTX2 hypoblast-like domain.

Submission Date

2024-10-04

Opened Date

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Release Date

2025-04-21

Update Date

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Kind

License

Project URL

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Project DOI

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Metadata Version

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Template Version

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Funding Information

This work was supported by MEXT KAKENHI (grant numbers: JP16H02465, JP15H02360, JP20H05361, JP20H05762, JP21K19189, JP23K18340) and AMED (grant numbers: JP21bm0704035, JP21bm0104001, JP21gm1310011, JP23bm1323001) to Y.T.; MEXT KAKENHI (grant number: JP19H03418) and the JST FOREST Program (grant number: JPMJFR206C), JST CREST (grant number: JPMJCR2023) and AMED (grant numbers: JP17gm1110004, JP19gm1310002, JP19bm0704032, JP21bm0104001, JP21gm1310011) to T.Y.; MEXT KAKENHI (grant numbers: JP15H02360, JP19H05759) to E.S.; AMED (grant number: JP22bm1004002) to H.N.; the Takeda Science Foundation to Y.T.; the Naito Foundation to Y.T.; the Mochida Memorial Foundation for Medical and Pharmaceutical Research to Y.T.; and the Astellas Foundation for Research on Metabolic Disorders to Y.T.

Total

16 files / 3.3 GB

Image datasets

zipped 8 files / 1.4 GB, raw image 8 files / 1.9 GB, total 16 files / 3.3 GB

Quantitative datasets

zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes

Organism

Strain

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Cell

Cell line

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MeSH

Anatomical Entity

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UBERON

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Biological Process

Cellular Component

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Molecular Function

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Medical Subject Headings

Method involved in biological imaging