369-Moriyama-deuterateRaman
Author reviewedMultiple deuteration of triphenylphosphine and live-cell Raman imaging
of deuterium-incorporated Mito-Q
Project ID
369-Moriyama-deuterateRaman
Project SID
369
Title
Multiple deuteration of triphenylphosphine and live-cell Raman imaging
of deuterium-incorporated Mito-Q
Description
All aromatic C–H bonds of triphenylphosphine (PPh3) were efficiently replaced by C–D bonds using Ru/C and Ir/C co-catalysts in 2-PrOH and D2O, an inexpensive deuterium source. Furthermore, non-radioactive and safe deuterium-incorporated Mito-Q (drug candidate) was prepared from deuterated PPh3 and used for the live-cell Raman imaging to evaluate the mitochondrial uptake.
Submission Date
2024-07-25
Opened Date
-
Release Date
2025-01-31
Update Date
-
Kind
Image datasets
License
Project URL
-
Project DOI
-
Metadata Version
-
Template Version
-
Funding Information
This study was partially supported by JSPS (MEXT grant in aid-for transformative research areas (B) Deuterium Science) KAKENHI Grant Number 20H05738 (for Y.S.), and Life Science and Drug Discovery (Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS)) from AMED under Grant Number 23ama121054 (for Y.S.).
Total
18 files / 266.2 KB
Image datasets
zipped 3 files / 114.6 KB, raw image 15 files / 151.7 KB, total 18 files / 266.2 KB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Biosample
Organism
Homo sapiens
(
NCBITaxon:9606
)
Strain
-
Cell
-
Cell line
HeLa Cell
(
CLO:0003684
)
MeSH
HeLa Cells
Mitochondria
Ontology
Anatomical Entity
-
UBERON
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Biological Process
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Cellular Component
mitochondrion
(
GO:0005739
)
Molecular Function
-
Medical Subject Headings
HeLa Cells
Mitochondria
Imaging Method
Method involved in biological imaging
inelastic scattering of photons (Raman scattering)
(
FBbi:00000589
)
Paper DOI / Paper URL
People
Contact
Yoshinari Sawama
Osaka University
Graduate School of Pharmaceutical Sciences
-
1-6, Yamada-oka, Suita, Osaka 565-0871, Japan.
Imaging dataset contributor
Shogo Moriyama
Daiki Shibata
Hiroyuki Yamakoshi
Shinji Kajimoto
Takakazu Nakabayashi
Quantitative dataset contributor
-
Image datasets
3
Quantitative datasets
0
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
Live-cell Raman images of HeLa cells using a deuterium-incorporated compound, TPMP-d15
Live-cell Raman images of HeLa cells using a deuterium-incorporated compound, Mito-Q-d15
Live-cell Raman images of HeLa cells using low concentration of deuterium-incorporated compound, TPMP-d15