Project
369-Moriyama-deuterateRaman
Title
Multiple deuteration of triphenylphosphine and live-cell Raman imaging
of deuterium-incorporated Mito-Q
Description
All aromatic C–H bonds of triphenylphosphine (PPh3) were efficiently replaced by C–D bonds using Ru/C and Ir/C co-catalysts in 2-PrOH and D2O, an inexpensive deuterium source. Furthermore, non-radioactive and safe deuterium-incorporated Mito-Q (drug candidate) was prepared from deuterated PPh3 and used for the live-cell Raman imaging to evaluate the mitochondrial uptake.
Funding
This study was partially supported by JSPS (MEXT grant in aid-for transformative research areas (B) Deuterium Science) KAKENHI Grant Number 20H05738 (for Y.S.), and Life Science and Drug Discovery (Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS)) from AMED under Grant Number 23ama121054 (for Y.S.).
Title
Live-cell Raman images of HeLa cells using low concentration of deuterium-incorporated compound, TPMP-d15
Description
Raman images of HeLa cells treated with 2 micromolar TPMP-d15 (2251-2322 cm^{-1}), which is deuterated methyltri phenylphosphonium iodide. The images of 60 min after TPMP-d15 treatment are shown. The Raman images of cytochrome c (739-759 cm^{-1}), and their merged image is also reposited.
Description
HeLa cells treated with deuterium-incorporated compound.
Gene ontology: Biological processes
Gene ontology: Cellular components
Gene ontology: Molecular functions
Microscopy types
inelastic scattering of photons (Raman scattering) (
FBbi:00000589)
Visualization methods
organelle-specific probe
Contrast enhancing methods
Resolution enhancing methods
Sample preparation methods
living tissue