366-Ebata-TERTlocalize
Author reviewedTime-lapse images showing the role of mitochondrial TERT during apoptosis
Project ID
366-Ebata-TERTlocalize
Project SID
366
Title
Time-lapse images showing the role of mitochondrial TERT during apoptosis
Description
Telomerase reverse transcriptase (TERT) is a protein that catalyzes the reverse transcription of telomere elongation. TERT is also expected to play a non-canonical role beyond telomere lengthening since it localizes not only in the nucleus but also in mitochondria, where telomeres do not exist. Several studies have reported that mitochondrial TERT regulates apoptosis induced by oxidative stress. However, there is still some controversy as to whether mitochondrial TERT promotes or inhibits apoptosis, mainly due to the lack of information on changes in TERT distribution in individual cells over time. Here, the authors simultaneously detected apoptosis and TERT localization after oxidative stress in individual HeLa cells by live-cell tracking. Single-cell tracking revealed that the stress-induced accumulation of TERT in mitochondria caused apoptosis, but that accumulation increased over time until cell death. The results suggest a new model in which mitochondrial TERT has two opposing effects at different stages of apoptosis: it predetermines apoptosis at the first stage of cell-fate determination, but also delays apoptosis at the second stage. As such, their data support a model that integrates the two opposing hypotheses on mitochondrial TERT’s effect on apoptosis. Furthermore, detailed statistical analysis of TERT mutations, which have been predicted to inhibit TERT transport to mitochondria, revealed that these mutations suppress apoptosis indepen- dent of mitochondrial localization of TERT. Together, these results imply that the non-canonical functions of TERT affect a wide range of mitochondria-dependent and mitochondria-independent apoptosis pathways.
Submission Date
2024-07-26
Opened Date
-
Release Date
2024-12-24
Update Date
-
Kind
Image datasets
License
Project URL
-
Project DOI
-
Metadata Version
-
Template Version
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Funding Information
This work was supported by the Japan Society for the Promotion of Science Grant-in-Aid for Japan Society for the Promotion of Science (JSPS) Fellows (JP21J10299) to HE and by JSPS KAKENHI (18K06147, 19H05379, and 21H00387) to TS.
Biosample
Organism
Homo sapiens
(
NCBITaxon:9606
)
Strain
-
Cell
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Cell line
HeLa cell
(
CLO:0003684
)
MeSH
Ontology
Anatomical Entity
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UBERON
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Biological Process
regulation of apoptotic process
(
GO:0042981
)
Cellular Component
mitochondrion, nucleus
(
GO:0005634
)
Molecular Function
telomerase activity
(
GO:0003720
)
Medical Subject Headings
Imaging Method
Method involved in biological imaging
fluorescence microscopy
(
FBbi:00000246
)
time lapse microscopy
(
FBbi:00000249
)
Paper DOI / Paper URL
People
Contact
Tomohiro Shima
The University of Tokyo
Department of Biological Sciences, Graduate School of Science
-
-
Sotaro Uemura
The University of Tokyo
Department of Biological Sciences, Graduate School of Science
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-
Imaging dataset contributor
Hiroshi Ebata (The University of Tokyo)
Quantitative dataset contributor
-
Image datasets
6
Quantitative datasets
0
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
Time-lapse images of HeLa cells with or without expressing the TERT constructs during apoptosis
Time-lapse images of HeLa cells with or without expressing the TERT constructs during apoptosis
Time-lapse images of HeLa cells with or without expressing the TERT constructs during apoptosis
Time-lapse images showing the translocation of mVenus-TERT in HeLa cells during apoptosis induced by oxidative stress
Time-lapse images showing the translocation of mVenus-TERT in HeLa cells during apoptosis induced by oxidative stress
Time-lapse images showing the translocation of mVenus-TERT in HeLa cells during apoptosis induced by oxidative stress