329-Nelson-Chemotaxis
Author reviewedAmmonium chemotaxis
of Chlamydomonas reinhardtii
Project ID
329-Nelson-Chemotaxis
Project SID
329
Title
Ammonium chemotaxis
of Chlamydomonas reinhardtii
Description
The mechanisms governing chemotaxis in Chlamydomonas reinhardtii are largely unknown compared to those regulating phototaxis despite equal importance on the migratory response in the ciliated microalga. To study chemotaxis, the authors made a simple modification to a conventional Petri dish assay. Using the assay, a novel mechanism governing Chlamydomonas ammonium chemotaxis was revealed. First, they found that light exposure enhances the chemotactic response of wild‐type Chlamydomonas strains, yet phototaxis‐incompetent mutant strains, eye3-2 and ptx1, exhibit normal chemotaxis. This suggests that Chlamydomonas transduces the light signal pathway in chemotaxis differently from that in phototaxis. Second, we found that Chlamydomonas collectively migrate during chemotaxis but not phototaxis. Collective migration during chemotaxis is not clearly observed when the assay
is conducted in the dark. Third, the Chlamydomonas strain CC‐124 carrying agg1−, the AGGREGATE1 gene (AGG1) null mutation, exhibited a more robust collective migratory response than strains carrying the wild‐type AGG1 gene. The expression of a recombinant AGG1 protein in the CC‐124 strain suppressed this collective migration during chemotaxis.
Submission Date
2024-04-11
Opened Date
-
Release Date
2025-01-31
Update Date
-
Kind
Image datasets
License
Project URL
-
Project DOI
-
Metadata Version
-
Template Version
-
Funding Information
This research was conducted with funds from Louisiana Economic Development Assistantship and LSU LIFT2 Fund for N.K. Japan Society for the Promotion of Science KAKENHI Grants 21H00420, 22H02642 and 22H05674 for K.W.
Total
186 files / 926.4 MB
Image datasets
zipped 33 files / 462.5 MB, raw image 153 files / 463.9 MB, total 186 files / 926.4 MB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Biosample
Organism
Chlamydomonas reinhardtii
(
NCBITaxon:3055
)
Strain
CC-124, CC-125
(
https://www.chlamycollection.org/product/cc-124-wild-type-mt-137c/
)
Cell
-
Cell line
-
MeSH
-
Ontology
Anatomical Entity
-
UBERON
-
Biological Process
cell chemotaxis
(
GO:0060326
)
Cellular Component
-
Molecular Function
-
Medical Subject Headings
-
Imaging Method
Method involved in biological imaging
macroscopy
microscopy
Paper DOI / Paper URL
People
Contact
Naohiro Kato
Louisiana State University
Department of Biological Sciences
-
-
Imaging dataset contributor
Gabela Nelson (Louisiana State University)
Alexis Strain (Louisiana State University)
Quantitative dataset contributor
-
Image datasets
33
Quantitative datasets
0
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
Time series images of the ammonium chemotaxis of Chlamydomonas CC-124
Time series images of the ammonium chemotaxis of Chlamydomonas CC-124
Time series images of the ammonium chemotaxis of Chlamydomonas CC-124
Time series images of the ammonium chemotaxis of two transgenic lines of Chlamydomonas CC-124
Time series images of the ammonium chemotaxis of two transgenic lines of Chlamydomonas CC-124
Time series images of the ammonium chemotaxis of two transgenic lines of Chlamydomonas CC-124
The movie of the ammonium chemotaxis of Chlamydomonas CC-124
Time movie of the collective migration of Chlamydomonas CC-124
The movie of the ammonium chemotaxis of wild type and two transgenic lines of Chlamydomonas CC-124
Chlamydomonas reinhardtii
(
NCBITaxon:3055
)
Time series images of the ammonium chemotaxis of Chlamydomonas CC-124 and CC-125, which were cultured autotrophically and assayed in a medium not containing acetate
Chlamydomonas reinhardtii
(
NCBITaxon:3055
)
Time series images of the ammonium chemotaxis of Chlamydomonas CC-124 and CC-125, which were cultured autotrophically and assayed in a medium not containing acetate
Chlamydomonas reinhardtii
(
NCBITaxon:3055
)
Time series images of the ammonium chemotaxis of Chlamydomonas CC-124 and CC-125, which were cultured autotrophically and assayed in a medium not containing acetate
Time series images of the collected cellular migration of Chlamydomonas CC-125
Time series images of the collected cellular migration of Chlamydomonas CC-125
Time series images of the collected cellular migration of Chlamydomonas CC-125
Time series images of the collected cellular migration of Chlamydomonas mutant, amt4- (CC-4042)
Time series images of the collected cellular migration of Chlamydomonas mutant, amt4- (CC-4042)
Time series images of the collected cellular migration of Chlamydomonas mutant, amt4- (CC-4042)
Chlamydomonas reinhardtii
(
NCBITaxon:3055
)
Time series images of the collected cellular migration of Chlamydomonas mutant, eye3-2 (CC-4316)
Chlamydomonas reinhardtii
(
NCBITaxon:3055
)
Time series images of the collected cellular migration of Chlamydomonas mutant, eye3-2 (CC-4316)
Chlamydomonas reinhardtii
(
NCBITaxon:3055
)
Time series images of the collected cellular migration of Chlamydomonas mutant, eye3-2 (CC-4316)
Time series images of the collected cellular migration of Chlamydomonas mutant, pf14 (CC-613)
Time series images of the collected cellular migration of Chlamydomonas mutant, pf14 (CC-613)
Time series images of the collected cellular migration of Chlamydomonas mutant, pf14 (CC-613)
Time series images of the collected cellular migration of Chlamydomonas mutant, pf18 (CC-1036)
Time series images of the collected cellular migration of Chlamydomonas mutant, pf18 (CC-1036)
Time series images of the collected cellular migration of Chlamydomonas mutant, pf18 (CC-1036)
Time series images of the collected cellular migration of Chlamydomonas mutant, ptx1 (CC- 2894)
Time series images of the collected cellular migration of Chlamydomonas mutant, ptx1 (CC- 2894)
Time series images of the collected cellular migration of Chlamydomonas mutant, ptx1 (CC- 2894)
Time series images of the ammonium chemotaxis of Chlamydomonas CC-124 at low density
Time series images of the ammonium chemotaxis of Chlamydomonas CC-124 at low density
Time series images of the ammonium chemotaxis of Chlamydomonas CC-124 at low density