SSBD:database v3

329-Nelson-Chemotaxis

OMERO gallery,
OMERO index

Project

Title
Ammonium chemotaxis of Chlamydomonas reinhardtii
Description
The mechanisms governing chemotaxis in Chlamydomonas reinhardtii are largely unknown compared to those regulating phototaxis despite equal importance on the migratory response in the ciliated microalga. To study chemotaxis, the authors made a simple modification to a conventional Petri dish assay. Using the assay, a novel mechanism governing Chlamydomonas ammonium chemotaxis was revealed. First, they found that light exposure enhances the chemotactic response of wild‐type Chlamydomonas strains, yet phototaxis‐incompetent mutant strains, eye3-2 and ptx1, exhibit normal chemotaxis. This suggests that Chlamydomonas transduces the light signal pathway in chemotaxis differently from that in phototaxis. Second, we found that Chlamydomonas collectively migrate during chemotaxis but not phototaxis. Collective migration during chemotaxis is not clearly observed when the assay is conducted in the dark. Third, the Chlamydomonas strain CC‐124 carrying agg1−, the AGGREGATE1 gene (AGG1) null mutation, exhibited a more robust collective migratory response than strains carrying the wild‐type AGG1 gene. The expression of a recombinant AGG1 protein in the CC‐124 strain suppressed this collective migration during chemotaxis.
License
CC BY
Released at
Oct. 20, 2025, midnight
Updated at
Funding
This research was conducted with funds from Louisiana Economic Development Assistantship and LSU LIFT2 Fund for N.K. Japan Society for the Promotion of Science KAKENHI Grants 21H00420, 22H02642 and 22H05674 for K.W.
# of image datasets
33

Papers

Papers
Paper information: Nelson G, Strain A, Isu A, Rahnama A, Wakabayashi KI, Melvin AT, Kato N. Cells collectively migrate during ammonium chemotaxis in Chlamydomonas reinhardtii. Sci Rep. 2023 Jul 4;13(1):10781,
Pubmed ID: 37402785,
PMCID: PMC10319813,
DOI: https://doi.org/10.1038/s41598-023-36818-6,
URL: https://pubmed.ncbi.nlm.nih.gov/37402785/

Contacts

Contacts
Naohiro Kato (Contact) , Department of Biological Sciences , Louisiana State University
Gabela Nelson (Imaging Contributor) , Department of Biological Sciences , Louisiana State University
Alexis Strain (Imaging Contributor) , Department of Biological Sciences , Louisiana State University

Biosamples

Organisms
Chlamydomonas reinhardtii (NCBI:txid3055)
Strains
CC-125
CC-613
CC-124
CC-4042
CC-4316
CC-124, CC-125
CC-1036
CC-2894
Cells

Cell lines

Ontologies

UBERON
Anatomical entities
GO: Biological processes
cell chemotaxis (GO:0060326) (GO:0060326)
GO: Cellular components
GO: Molecular functions

Genetics

Gene names
Protein names
Genetic methods
electroporation (http%253A%252F%252Fpurl.allotrope.org%252Fontologies%252Fprocess%2523AFP_0001370)
electroporation (http%253A%252F%252Fpurl.allotrope.org%252Fontologies%252Fprocess%2523AFP_0001367)
electroporation (http%253A%252F%252Fpurl.allotrope.org%252Fontologies%252Fprocess%2523AFP_0001368)
electroporation (http%253A%252F%252Fpurl.allotrope.org%252Fontologies%252Fprocess%2523AFP_0001369)
Protein tags
Probes
Oligo Primer
Genotype

Treatments

Reagent or Compound
Concentration
Fold Dilution

Imaging Methods

Dimensions
3832x2874x1x3x1, 4032x3024x1x3x1, 3865x2899x1x3x1, NA, 3919x2939xXXx3xXX, 3804x2853x1x3x1, 3919x2939x1x3x1, 3915x2936x1x3x1, 3974x2980x1x1x1, 4032x3024x1x1x1, 4032x3024xX1x1x1, 3907x2930x1x4x1, 4032x3024x1x2x1,
X scales
NA,
Y scales
NA,
Z scales
NA,
T scales
NA, 5 minutes, 2 seconds,
Channels
1 channel,
Microscopy types
Detection methods
Visualization methods
Illumination methods
Sources of contrast
Contrast enhancing methods
Resolution enhancing methods
Imaged parameters
Sample preparation methods

Instruments

Body
Model
Light source
Detector
Objective
Filter set
Dichroic
Body
LEICA inverted microscope (DMI6000 B)
Model
Light source
Transmission
Detector
HAYER HY-2307
Objective
Leica HI PLAN 20x/0,40 POL
Filter set
Dichroic

Datasets

Dataset name
Organism
Gene / Protein
Dimensions
File size
SSBD:OMERO
Download
Fig1A_chemotaxis_1
Chlamydomonas reinhardtii (NCBI:txid3055)
4032x3024x1x1x1
12.6MB
Gallery, Index
zip
Fig1A_chemotaxis_2
Chlamydomonas reinhardtii (NCBI:txid3055)
4032x3024x1x1x1
16.8MB
Gallery, Index
zip
Fig1A_chemotaxis_3
Chlamydomonas reinhardtii (NCBI:txid3055)
4032x3024x1x1x1
15.7MB
Gallery, Index
zip
MovieS1_chemotaxis
Chlamydomonas reinhardtii (NCBI:txid3055)
NA
1MB
Gallery, Index
zip
SupplFig6_chemotaxis_NoAcetate_1
Chlamydomonas reinhardtii (NCBI:txid3055)
4032x3024xX1x1x1
26.2MB
Gallery, Index
zip
SupplFig6_chemotaxis_NoAcetate_2
Chlamydomonas reinhardtii (NCBI:txid3055)
4032x3024x1x2x1
29.4MB
Gallery, Index
zip
SupplFig6_chemotaxis_NoAcetate_3
Chlamydomonas reinhardtii (NCBI:txid3055)
4032x3024x1x3x1
25.2MB
Gallery, Index
zip
SupplFig7_chemo_pf14_1
Chlamydomonas reinhardtii (NCBI:txid3055)
3832x2874x1x3x1
18.9MB
Gallery, Index
zip
SupplFig7_chemo_pf14_2
Chlamydomonas reinhardtii (NCBI:txid3055)
3907x2930x1x4x1
19.9MB
Gallery, Index
zip
SupplFig7_chemo_pf14_3
Chlamydomonas reinhardtii (NCBI:txid3055)
3915x2936x1x3x1
17.8MB
Gallery, Index
zip
SupplFig7_chemo_pf18_1
Chlamydomonas reinhardtii (NCBI:txid3055)
3804x2853x1x3x1
21MB
Gallery, Index
zip
SupplFig7_chemo_pf18_2
Chlamydomonas reinhardtii (NCBI:txid3055)
4032x3024x1x3x1
17.8MB
Gallery, Index
zip
SupplFig7_chemo_pf18_3
Chlamydomonas reinhardtii (NCBI:txid3055)
4032x3024x1x3x1
22MB
Gallery, Index
zip
SupplFig7_chemo_eye3-2_1
Chlamydomonas reinhardtii (NCBI:txid3055)
4032x3024x1x3x1
15.7MB
Gallery, Index
zip
SupplFig7_chemo_eye3-2_2
Chlamydomonas reinhardtii (NCBI:txid3055)
4032x3024x1x3x1
15.7MB
Gallery, Index
zip
SupplFig7_chemo_eye3-2_3
Chlamydomonas reinhardtii (NCBI:txid3055)
4032x3024x1x3x1
15.7MB
Gallery, Index
zip
SupplFig7_chemo_ptx1_1
Chlamydomonas reinhardtii (NCBI:txid3055)
3865x2899x1x3x1
15.7MB
Gallery, Index
zip
SupplFig7_chemo_ptx1_2
Chlamydomonas reinhardtii (NCBI:txid3055)
3919x2939x1x3x1
13.6MB
Gallery, Index
zip
SupplFig7_chemo_ptx1_3
Chlamydomonas reinhardtii (NCBI:txid3055)
4032x3024x1x3x1
13.6MB
Gallery, Index
zip
SupplFig7_chemo_amt4_1
Chlamydomonas reinhardtii (NCBI:txid3055)
4032x3024x1x3x1
25.2MB
Gallery, Index
zip
SupplFig7_chemo_amt4_2
Chlamydomonas reinhardtii (NCBI:txid3055)
4032x3024x1x3x1
23.1MB
Gallery, Index
zip
SupplFig7_chemo_amt4_3
Chlamydomonas reinhardtii (NCBI:txid3055)
4032x3024x1x3x1
23.1MB
Gallery, Index
zip
SupplFig7_chemo_CC125_1
Chlamydomonas reinhardtii (NCBI:txid3055)
3865x2899x1x3x1
16.8MB
Gallery, Index
zip
SupplFig7_chemo_CC125_2
Chlamydomonas reinhardtii (NCBI:txid3055)
3919x2939xXXx3xXX
12.8MB
Gallery, Index
zip
SupplFig7_chemo_CC125_3
Chlamydomonas reinhardtii (NCBI:txid3055)
4032x3024x1x3x1
12.6MB
Gallery, Index
zip
MovieS2_migrate
Chlamydomonas reinhardtii (NCBI:txid3055)
NA
10.3MB
Gallery, Index
zip
SupplFig9_LowDensity_1
Chlamydomonas reinhardtii (NCBI:txid3055)
4032x3024x1x3x1
14.7MB
Gallery, Index
zip
SupplFig9_LowDensity_2
Chlamydomonas reinhardtii (NCBI:txid3055)
4032x3024x1x3x1
13.6MB
Gallery, Index
zip
SupplFig9_LowDensity_3
Chlamydomonas reinhardtii (NCBI:txid3055)
4032x3024x1x3x1
14.7MB
Gallery, Index
zip
Fig5_chemo_transgenic_1
Chlamydomonas reinhardtii (NCBI:txid3055)
agg1 / AGGREGATE1
3974x2980x1x1x1
18.9MB
Gallery, Index
zip
Fig5_chemo_transgenic_2
Chlamydomonas reinhardtii (NCBI:txid3055)
agg1 / AGGREGATE1
3974x2980x1x1x1
18.9MB
Gallery, Index
zip
Fig5_chemo_transgenic_3
Chlamydomonas reinhardtii (NCBI:txid3055)
agg1 / AGGREGATE1
3974x2980x1x1x1
18.9MB
Gallery, Index
zip
MovieS3_transgenic
Chlamydomonas reinhardtii (NCBI:txid3055)
agg1 / AGGREGATE1
NA
2.6MB
Gallery, Index
zip