Dataset Fig5_chemo_transgenic_2
Project
329-Nelson-Chemotaxis
Title
Ammonium chemotaxis
of Chlamydomonas reinhardtii
Description
The mechanisms governing chemotaxis in Chlamydomonas reinhardtii are largely unknown compared to those regulating phototaxis despite equal importance on the migratory response in the ciliated microalga. To study chemotaxis, the authors made a simple modification to a conventional Petri dish assay. Using the assay, a novel mechanism governing Chlamydomonas ammonium chemotaxis was revealed. First, they found that light exposure enhances the chemotactic response of wild‐type Chlamydomonas strains, yet phototaxis‐incompetent mutant strains, eye3-2 and ptx1, exhibit normal chemotaxis. This suggests that Chlamydomonas transduces the light signal pathway in chemotaxis differently from that in phototaxis. Second, we found that Chlamydomonas collectively migrate during chemotaxis but not phototaxis. Collective migration during chemotaxis is not clearly observed when the assay
is conducted in the dark. Third, the Chlamydomonas strain CC‐124 carrying agg1−, the AGGREGATE1 gene (AGG1) null mutation, exhibited a more robust collective migratory response than strains carrying the wild‐type AGG1 gene. The expression of a recombinant AGG1 protein in the CC‐124 strain suppressed this collective migration during chemotaxis.
Funding
This research was conducted with funds from Louisiana Economic Development Assistantship and LSU LIFT2 Fund for N.K. Japan Society for the Promotion of Science KAKENHI Grants 21H00420, 22H02642 and 22H05674 for K.W.
Title
Time series images of the ammonium chemotaxis of two transgenic lines of Chlamydomonas CC-124
Description
Time series images of the ammonium chemotaxis of two transgenic lines of Chlamydomonas CC-124, in which the recombinant AGG1 protein was expressed. Time series of 0h, 3h, 6h, 12h, 24h.
Description
Two transgenic lines of Chlamydomonas CC-124, in which the recombinant AGG1 protein was expressed.
Intrinsic variables
Transgenic lines of Chlamydomonas CC-124, in which the recombinant AGG2 protein was expressed.
Gene ontology: Biological processes
Gene ontology: Cellular components
Gene ontology: Molecular functions
Dimensions
3974x2980x1x1x1
Microscopy types
(macroscopy)
Contrast enhancing methods
Resolution enhancing methods
Sample preparation methods