Dataset 337-Isoe-BrainRegion/Video1_ReferenceBrain
Project
337-Isoe-BrainRegion
Title
3D medaka brain visualized clonally related units in the pallium
Description
The dorsal telencephalon (i.e. the pallium) exhibits high anatomical diversity across vertebrate classes. The non-mammalian dorsal pallium accommodates various compartmentalized structures among species. The developmental, functional, and evolutional diversity of the dorsal pallium remain unillustrated. Here, the authors analyzed the structure and epigenetic landscapes of cell lineages in the telencephalon of medaka fish (Oryzias latipes) that possesses a clearly delineated dorsal pallium (Dd2). They found that pallial anatomical regions, including Dd2, are formed by mutually exclusive clonal units, and that each pallium compartment exhibits a distinct epigenetic landscape.
Funding
NIBB Collaborative Research Program 16-522 (HT), KAKENHI 16H06987 (YI), KAKENHI 16KT0072 (HT), KAKENHI 20K20303 (YK), JP21K06013 (RN), Grant-in-Aid for Scientific Research on Innovative Areas JP21H00245 (RN), 18-513 (HT), 17-513 (HT)
Dataset
Video1_ReferenceBrain
Title
Z-stack images of horizontal section of the reference medaka brain stained with DAPI
Description
Z-series images of horizontal section of the reference medaka brain obtained by light sheet microscopy. The authors observed three-dimensional distribution of cell nuclei by DAPI staining and tissue clearing, and redefined the anatomical regions in the adult medaka (2-month-old) telencephalon.
File separation method
NA
Gene ontology: Biological processes
Gene ontology: Cellular components
Gene ontology: Molecular functions
Molecular biological information
Dimensions
300x250x319x1x1
Contrast enhancing methods
Resolution enhancing methods
Sample preparation methods