Dataset Video1_ReferenceBrain
Project
337-Isoe-BrainRegion
Title
3D medaka brain visualized clonally related units in the pallium
Description
The dorsal telencephalon (i.e. the pallium) exhibits high anatomical diversity across vertebrate classes. The non-mammalian dorsal pallium accommodates various compartmentalized structures among species. The developmental, functional, and evolutional diversity of the dorsal pallium remain unillustrated. Here, the authors analyzed the structure and epigenetic landscapes of cell lineages in the telencephalon of medaka fish (Oryzias latipes) that possesses a clearly delineated dorsal pallium (Dd2). They found that pallial anatomical regions, including Dd2, are formed by mutually exclusive clonal units, and that each pallium compartment exhibits a distinct epigenetic landscape.
Funding
NIBB Collaborative Research Program 16-522 (HT), KAKENHI 16H06987 (YI), KAKENHI 16KT0072 (HT), KAKENHI 20K20303 (YK), JP21K06013 (RN), Grant-in-Aid for Scientific Research on Innovative Areas JP21H00245 (RN), 18-513 (HT), 17-513 (HT)
Title
Z-stack images of horizontal section of the reference medaka brain stained with DAPI
Description
Z-series images of horizontal section of the reference medaka brain obtained by light sheet microscopy. The authors observed three-dimensional distribution of cell nuclei by DAPI staining and tissue clearing, and redefined the anatomical regions in the adult medaka (2-month-old) telencephalon.
Description
The wild type medaka brain.
Gene ontology: Biological processes
Gene ontology: Cellular components
Gene ontology: Molecular functions
Dimensions
300x250x319x1x1
Microscopy types
(light-sheet microscopy)
Detection methods
charge coupled device (CCD)
Visualization methods
DAPI staining
Illumination methods
charge coupled device (CCD)
Contrast enhancing methods
Resolution enhancing methods
Sample preparation methods
whole mounted tissue
Light source
Argon-krypton laser
Objective
Zeiss Achroplan 20x/0.5
Filter set
RazorEdge RU 488, 568, Semrock