Locomotion of mig-17/ ADAMTS and chondroitin related mutants
Description
These movies represents locomotion of aged C. elegans at day1, 3, 5, 7, and 9 adult on OP50 bacteria after tap stimuli in wild type N2, mig-17 (k174) mutamts, mig-22 (k185) mutants, sqv-5 (k175) mutants, mig-17 (k174) mig-22 (k185) double mutants, and sqv-5 (k175) mig-22 (k185) double mutants. The anomunt of chondroitin in mig-22 (k185) mutants is two times conmpare to wild type. On the other hand, that in sqv-5 (k175) mutants is one tenth conmpare to wild type. Day 1 adult that did not have egg was collected. Then, animals are transfered to new plate at each two days until their behavior recoreded. In case of sqv-5 mutants, since some of them are sterile, we collected L4 larve at first. Then, we used these animals as day 1 adult at the next day.
This work was supported by a Grant-in- Aid for Research Activity Start-up by the Ministry of Education, Culture, Sports, Science and Technology to YS(22K20658) and by the Naito Grant for the advancement of natural science to KN