Hiroyuki Yamakoshi, Daiki Shibata, Kazuki Bando, Shinji Kajimoto, Aki Kohyama, Syusuke Egoshi, Kosuke Dodo, Yoshiharu Iwabuchi, Mikiko Sodeoka, Katsumasa Fujita, Takakazu Nakabayashi (2023) Ratiometric analysis of reversible thia-Michael reactions using nitrile-tagged molecules by Raman microscopy., Chemical communications (Cambridge, England)
Published in 2023 Nov 21 (Electronic publication in Nov. 21, 2023, midnight )
(Abstract) Ratiometric Raman analysis of reversible thia-Michael reactions was achieved using alpha-cyanoacrylic acid (alphaCNA) derivatives. Among alphaCNAs, the smallest derivative, ThioRas (molecular weight: 167 g mol(-1)), and its glutathione adduct were simultaneously detected in various subcellular locations using Raman microscopy.